دورية أكاديمية
The scavenger receptors SRA-1 and SREC-I cooperate with TLR2 in the recognition of the hepatitis C virus non-structural protein 3 by dendritic cells
| العنوان: | The scavenger receptors SRA-1 and SREC-I cooperate with TLR2 in the recognition of the hepatitis C virus non-structural protein 3 by dendritic cells |
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| المؤلفون: | Beauvillain, Céline, Meloni, Francesca, Sirard, Jean-Claude, Blanchard, Simon, Jarry, Ulrich, Scotet, Mari, Magistrelli, Giovanni, Delneste, Yves, Barnaba, Vincenzo, Jeannin, Pascale |
| المساهمون: | Centre de Recherche en Cancérologie Nantes-Angers (CRCNA), Université d'Angers (UA)-Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Universitaire de Nantes = Nantes University Hospital (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Laboratoire d'Immunologie et d'Allergologie CHU Angers, Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM), Institut Pasteur, Fondation Cenci Bolognetti - Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Réseau International des Instituts Pasteur (RIIP), Interactions cellulaires et moléculaires des bactéries pathogènes avec l'hôte, Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Droit et Santé, Novimmune SA, This work was supported by institutional grants from Inserm and University of Angers and by grants from the Ligue contre le Cancer (équipe labellisée 2008–2010 and Comité départemental du Maine et Loire), Cancéropole Grand-Ouest and Région Pays de la Loire (project CIMATH). U.J. received a grant from the Association pour la Recherche contre le Cancer. |
| المصدر: | ISSN: 0168-8278. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | HCV NS3, TLR2, Scavenger receptors, Cross-presentation, Cooperation between innate receptors, Vaccine vector, MESH: Adaptor Proteins, Signal Transducing, MESH: Animals, MESH: CHO Cells, MESH: Cell Differentiation, MESH: Cricetulus, MESH: Cricetinae, MESH: Dendritic Cells, MESH: Hepacivirus, MESH: Endocytosis, MESH: Humans, MESH: Mice, MESH: Monocytes, MESH: Receptors, Scavenger, MESH: Myeloid Cells, MESH: Recombinant Proteins, MESH: Toll-Like Receptor 2, MESH: Scavenger Receptors, Class F, MESH: Transfection, MESH: Antigens, CD14, MESH: Viral Nonstructural Proteins |
| الوصف: | International audience ; BACKGROUNDS & AIMS: The hepatitis C virus NS3 protein is taken up by myeloid cells in a TLR2-independent manner and activates myeloid cells via TLR2. This study aimed to identify the endocytic receptor(s) involved in the uptake of NS3 by myeloid cells and its relation with TLR2. METHODS: Inhibitors and transfected cells were used to identify the nature of the NS3-binding receptors expressed by myeloid cells. The cooperation between scavenger receptors (SRs) and TLR2 in the NS3-mediated activation of myeloid cells was evaluated using inhibitors, cells from TLR2(-/-) mice, and confocal microscopy. The involvement of SRs in NS3 cross-presentation was evaluated in vitro using an NS3-specific human T-cell clone. RESULTS: We observed that SRs are the main binding structures for NS3 on myeloid cells and identified the SRs SRA-1 and SREC-I as endocytic receptors for NS3. Moreover, both SRs and TLR2 cooperate in NS3-induced myeloid cell activation. CONCLUSION: This study highlights a central role for SRs in NS3 uptake and cross-presentation, and demonstrates a tightly orchestrated cooperation between signalling and endocytic innate receptors in NS3 recognition. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/20338659; inserm-00926610; https://inserm.hal.science/inserm-00926610Test; https://inserm.hal.science/inserm-00926610/documentTest; https://inserm.hal.science/inserm-00926610/file/Beauvillain%20et%20al%202010.pdfTest; PUBMED: 20338659 |
| DOI: | 10.1016/j.jhep.2009.11.031 |
| الإتاحة: | https://doi.org/10.1016/j.jhep.2009.11.031Test https://inserm.hal.science/inserm-00926610Test https://inserm.hal.science/inserm-00926610/documentTest https://inserm.hal.science/inserm-00926610/file/Beauvillain%20et%20al%202010.pdfTest |
| رقم الانضمام: | edsbas.66D4B5B9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.jhep.2009.11.031 |
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