دورية أكاديمية
A cisacting diversification activator both necessary and sufficient for AID-mediated hypermutation. PLoS Genet 5
| العنوان: | A cisacting diversification activator both necessary and sufficient for AID-mediated hypermutation. PLoS Genet 5 |
|---|---|
| المؤلفون: | Artem Blagodatski, Vera Batrak, Sabine Schmidl, Ulrike Schoetz, Olph B. Caldwell, Jean-marie Buerstedde |
| المساهمون: | The Pennsylvania State University CiteSeerX Archives |
| المصدر: | ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/44/ce/PLoS_Genet_2009_Jan_9_5(1)_e1000332.tar.gz |
| سنة النشر: | 2009 |
| المجموعة: | CiteSeerX |
| مصطلحات موضوعية: | These authors contributed equally to this work |
| الوصف: | Hypermutation of the immunoglobulin (Ig) genes requires Activation Induced cytidine Deaminase (AID) and transcription, but it remains unclear why other transcribed genes of B cells do not mutate. We describe a reporter transgene crippled by hypermutation when inserted into or near the Ig light chain (IgL) locus of the DT40 B cell line yet stably expressed when inserted into other chromosomal positions. Step-wise deletions of the IgL locus revealed that a sequence extending for 9.8 kilobases downstream of the IgL transcription start site confers the hypermutation activity. This sequence, named DIVAC for diversification activator, efficiently activates hypermutation when inserted at non-Ig loci. The results significantly extend previously reported findings on AID-mediated gene diversification. They show by both deletion and insertion analyses that |
| نوع الوثيقة: | text |
| وصف الملف: | application/zip |
| اللغة: | English |
| العلاقة: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.358.6977Test |
| حقوق: | Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: | edsbas.643F25F8 |
| قاعدة البيانات: | BASE |
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