التفاصيل البيبلوغرافية
| العنوان: |
A cisacting diversification activator both necessary and sufficient for AID-mediated hypermutation. PLoS Genet 5 |
| المؤلفون: |
Artem Blagodatski, Vera Batrak, Sabine Schmidl, Ulrike Schoetz, Olph B. Caldwell, Jean-marie Buerstedde |
| المساهمون: |
The Pennsylvania State University CiteSeerX Archives |
| المصدر: |
ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/44/ce/PLoS_Genet_2009_Jan_9_5(1)_e1000332.tar.gz |
| سنة النشر: |
2009 |
| المجموعة: |
CiteSeerX |
| مصطلحات موضوعية: |
These authors contributed equally to this work |
| الوصف: |
Hypermutation of the immunoglobulin (Ig) genes requires Activation Induced cytidine Deaminase (AID) and transcription, but it remains unclear why other transcribed genes of B cells do not mutate. We describe a reporter transgene crippled by hypermutation when inserted into or near the Ig light chain (IgL) locus of the DT40 B cell line yet stably expressed when inserted into other chromosomal positions. Step-wise deletions of the IgL locus revealed that a sequence extending for 9.8 kilobases downstream of the IgL transcription start site confers the hypermutation activity. This sequence, named DIVAC for diversification activator, efficiently activates hypermutation when inserted at non-Ig loci. The results significantly extend previously reported findings on AID-mediated gene diversification. They show by both deletion and insertion analyses that |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/zip |
| اللغة: |
English |
| العلاقة: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.358.6977Test |
| حقوق: |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: |
edsbas.643F25F8 |
| قاعدة البيانات: |
BASE |
