دورية أكاديمية
Combined use of hard X-ray phase contrast imaging and X-ray fluorescence microscopy for sub-cellular metal quantification.
| العنوان: | Combined use of hard X-ray phase contrast imaging and X-ray fluorescence microscopy for sub-cellular metal quantification. |
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| المؤلفون: | Kosior, Ewelina, Bohic, Sylvain, Suhonen, Heikki, Ortega, Richard, Devès, Guillaume, Carmona, Asuncion, Marchi, Florence, Guillet, Jean Francois, Cloetens, Peter |
| المساهمون: | European Synchrotron Radiation Facility (ESRF), INSERM U836, équipe 6, Rayonnement synchrotron et recherche médicale, European Synchrotron Radiation Facility (ESRF)-Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre d'Etudes Nucléaires de Bordeaux Gradignan (CENBG), Université Sciences et Technologies - Bordeaux 1 (UB)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), CP, Physics department - UFR PHITEM, Université Joseph Fourier - Grenoble 1 (UJF)-Université Joseph Fourier - Grenoble 1 (UJF)-Surface Science Laboratory, European Synchrotron Radiation Facility (ESRF)-European Synchrotron Radiation Facility (ESRF), Surface Science Laboratory |
| المصدر: | ISSN: 1047-8477. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2012 |
| المجموعة: | Université Grenoble Alpes: HAL |
| مصطلحات موضوعية: | X-ray fluorescence, phase contrast imaging, synchrotron, trace elements, MESH: Algorithms, MESH: Animals, MESH: Reference Standards, MESH: Single-Cell Analysis, MESH: Trace Elements, MESH: X-Rays, MESH: Zinc, MESH: Calibration, MESH: Cell Nucleus, MESH: Microscopy, Fluorescence, Phase-Contrast, MESH: PC12 Cells, MESH: Particle Size, MESH: Potassium, MESH: Rats, [SDV.IB.MN]Life Sciences [q-bio]/Bioengineering/Nuclear medicine |
| الوصف: | International audience ; Hard X-ray fluorescence microscopy and magnified phase contrast imaging are combined to obtain quantitative maps of the projected metal concentration in whole cells. The experiments were performed on freeze dried cells at the nano-imaging station ID22NI of the European Synchrotron Radiation Facility (ESRF). X-ray fluorescence analysis gives the areal mass of most major, minor and trace elements; it is validated using a biological standard of known composition. Quantitative phase contrast imaging provides maps of the projected mass and is validated using calibration samples and through comparison with Atomic Force Microscopy and Scanning Transmission Ion Microscopy. Up to now, absolute quantification at the sub-cellular level was impossible using X-ray fluorescence microscopy but can be reached with the use of the proposed approach. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/22182730; inserm-00662829; https://inserm.hal.science/inserm-00662829Test; https://inserm.hal.science/inserm-00662829/documentTest; https://inserm.hal.science/inserm-00662829/file/Kosior_JSB-2011_Combined_use_of_hard.pdfTest; PUBMED: 22182730 |
| DOI: | 10.1016/j.jsb.2011.12.005 |
| الإتاحة: | https://doi.org/10.1016/j.jsb.2011.12.005Test https://inserm.hal.science/inserm-00662829Test https://inserm.hal.science/inserm-00662829/documentTest https://inserm.hal.science/inserm-00662829/file/Kosior_JSB-2011_Combined_use_of_hard.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.642523E5 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.jsb.2011.12.005 |
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