دورية أكاديمية
Human chromokinesins promote chromosome congression and spindle microtubule dynamics during mitosis
| العنوان: | Human chromokinesins promote chromosome congression and spindle microtubule dynamics during mitosis |
|---|---|
| المؤلفون: | Wandke, Cornelia, Barisic, Marin, Sigl, Reinhard, Rauch, Veronika, Wolf, Frank, Amaro, Ana C, Tan, Chia Huei, Pereira, Antonio J, Kutay, Ulrike, Maiato, Helder, Meraldi, Patrick, Geley, Stephan |
| المصدر: | ISSN: 0021-9525 ; The Journal of cell biology, vol. 198, no. 5 (2012) p. 847-863. |
| سنة النشر: | 2012 |
| المجموعة: | Université de Genève: Archive ouverte UNIGE |
| مصطلحات موضوعية: | info:eu-repo/classification/ddc/612, Anaphase/physiology, Animals, Calcium-Binding Proteins/metabolism, Cell Cycle Checkpoints/physiology, Cell Cycle Proteins/metabolism, Cell Line, Tumor, Chromosome Segregation/physiology, DNA-Binding Proteins/metabolism, HCT116 Cells, HEK293 Cells, HeLa Cells, Humans, Kinesin/metabolism, Kinetochores/metabolism, Metaphase/physiology, Microtubules/metabolism, Mitosis/physiology, Mitotic Spindle Apparatus/metabolism, Nuclear Proteins/metabolism, Repressor Proteins/metabolism, Xenopus, Xenopus Proteins/metabolism |
| الوصف: | Chromokinesins are microtubule plus end-directed motor proteins that bind to chromosome arms. In Xenopus egg cell-free extracts, Xkid and Xklp1 are essential for bipolar spindle formation but the functions of the human homologues, hKID (KIF22) and KIF4A, are poorly understood. By using RNAi-mediated protein knockdown in human cells, we find that only co-depletion delayed progression through mitosis in a Mad2-dependent manner. Depletion of hKID caused abnormal chromosome arm orientation, delayed chromosome congression, and sensitized cells to nocodazole. Knockdown of KIF4A increased the number and length of microtubules, altered kinetochore oscillations, and decreased kinetochore microtubule flux. These changes were associated with failures in establishing a tight metaphase plate and an increase in anaphase lagging chromosomes. Co-depletion of both chromokinesins aggravated chromosome attachment failures, which led to mitotic arrest. Thus, hKID and KIF4A contribute independently to the rapid and correct attachment of chromosomes by controlling the positioning of chromosome arms and the dynamics of microtubules, respectively. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/22945934; https://archive-ouverte.unige.ch/unige:28854Test; unige:28854 |
| الإتاحة: | https://doi.org/10.1083/jcb.201110060Test https://archive-ouverte.unige.ch/unige:28854Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.6390503C |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |