دورية أكاديمية
أعرض في EDS

A novel PCR protocol for detection and differentiation of neuropathogenic and non-neuropathogenic equid alphaherpesvirus 1

التفاصيل البيبلوغرافية
العنوان: A novel PCR protocol for detection and differentiation of neuropathogenic and non-neuropathogenic equid alphaherpesvirus 1
المؤلفون: Lechmann, Julia, Schoster, Angelika, Ernstberger, Martina, Fouché, Nathalie, Fraefel, Cornel, Bachofen, Claudia
المصدر: Lechmann, Julia; Schoster, Angelika; Ernstberger, Martina; Fouché, Nathalie; Fraefel, Cornel; Bachofen, Claudia (2019). A novel PCR protocol for detection and differentiation of neuropathogenic and non-neuropathogenic equid alphaherpesvirus 1. Journal of Veterinary Diagnostic Investigation, 31(5):696-703.
بيانات النشر: Sage Publications
سنة النشر: 2019
المجموعة: University of Zurich (UZH): ZORA (Zurich Open Repository and Archive
مصطلحات موضوعية: Equine Department, Department of Farm Animals, Institute of Virology, 570 Life sciences, biology, 630 Agriculture, General Veterinary, PCR, equid alphaherpesvirus 1, neuropathogenic, restriction enzyme analysis, sequencing
الوصف: Equid alphaherpesvirus 1 (EHV-1) infections can have a major impact on the horse industry and equine welfare by causing abortion or respiratory or neurologic disease. A single nucleotide polymorphism (A2254→G2254) in open reading frame (ORF) 30, encoding the catalytic subunit of the DNA polymerase, has been shown to be a strong predictive marker for neuropathogenicity. Given that a previously established real-time PCR (rtPCR) protocol yielded unsatisfactory results concerning determination of the EHV-1 genotype, we developed and evaluated a new conventional PCR protocol enabling identification of the genotype by sequencing and restriction enzyme analysis (REA). Thirty samples from horses with signs typical for EHV-1 infection were tested by rtPCR and our new conventional PCR. The results showed that compared to rtPCR, the conventional PCR protocol combined with sequencing and REA was more reliable concerning unambiguous determination of the EHV-1 genotype. Results of our new assay confirmed previous findings, according to which the non-neuropathogenic genotype A2254 is predominantly found in animals with fever, respiratory signs, and abortions or perinatal mortality, whereas the neuropathogenic genotype G2254 is primarily detected in animals suffering from neurologic disease. In some samples, results pointed towards coinfection with both genotypes. Further studies are required in order to elucidate the significance of infections with genotype A2254 and G2254 in neurologic and non-neurologic cases, respectively.
نوع الوثيقة: article in journal/newspaper
وصف الملف: application/pdf
اللغة: English
تدمد: 1040-6387
العلاقة: https://www.zora.uzh.ch/id/eprint/175314/1/Lechmann_2019_%28J_Vet_Diagnostic_Invest%29_A_novel_PCR_protocol_for_detection_and_differentiation_of_neuropathogenic_and_non-ne.pdfTest; info:pmid/31477001; urn:issn:1040-6387
DOI: 10.5167/uzh-175314
DOI: 10.1177/1040638719871975
الإتاحة: https://doi.org/10.5167/uzh-17531410.1177/1040638719871975Test
https://www.zora.uzh.ch/id/eprint/175314Test/
https://www.zora.uzh.ch/id/eprint/175314/1/Lechmann_2019_%28J_Vet_Diagnostic_Invest%29_A_novel_PCR_protocol_for_detection_and_differentiation_of_neuropathogenic_and_non-ne.pdfTest
حقوق: info:eu-repo/semantics/closedAccess
رقم الانضمام: edsbas.6317D337
قاعدة البيانات: BASE
الوصف
تدمد:10406387
DOI:10.5167/uzh-175314