دورية أكاديمية
Three-dimensional culture models to study glioblastoma — current trends and future perspectives
| العنوان: | Three-dimensional culture models to study glioblastoma — current trends and future perspectives |
|---|---|
| المؤلفون: | Vareecal Joseph, Justin, Blaavand, Mathilde S., Daubon, Thomas, Kruyt, Frank AE., Thomsen, Martin Kristian |
| المصدر: | Vareecal Joseph , J , Blaavand , M S , Daubon , T , Kruyt , F AE & Thomsen , M K 2021 , ' Three-dimensional culture models to study glioblastoma — current trends and future perspectives ' , Current Opinion in Pharmacology , vol. 61 , pp. 91-97 . https://doi.org/10.1016/j.coph.2021.08.019Test |
| سنة النشر: | 2021 |
| المجموعة: | Aarhus University: Research |
| مصطلحات موضوعية: | CRISPR, 3D culture, Human pluripotent stem cells (hPSCs), Glioblastoma, Organoid, EGF, Human pluripotent stem, PHENOTYPE, IDENTIFICATION, TUMORS, HUMAN BRAIN-DEVELOPMENT, CEREBRAL ORGANOIDS, DISEASE, GROWTH, STEM-CELL, BFGF, Humans, Tumor Microenvironment, Brain Neoplasms/genetics, Glioblastoma/drug therapy, Organoids |
| الوصف: | Glioblastoma (GBM) is the most prevalent form of primary malignant brain tumor in adults and remains almost invariably lethal owing to its aggressive and invasive nature. There have only been marginal improvements in its bleak survival rate of 12–15 months over the last four decades. The lack of preclinical models that efficiently recapitulate tumor biology and the tumor microenvironment is also in part responsible for the slow phase of translational GBM research. Emerging three-dimensional (3D) organoids and cell culture systems offer new and innovative possibilities for GBM modelling. These 3D models find their application to engineer the disease, screen drugs, establishing live biobank, and explore personalized therapy. Furthermore, these models can also be genetically modified by using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technology, which would allow one to study the specific role of key genes associated with gliomagenesis. Establishment of a coculture system with GBM cells to understand its invasive behavior is yet another major application of this model. Despite these merits, the organoid models also have certain limitations, including the absence of immune responses and vascular systems. In recent years, major progress has been made in the development and refinement of 3D models of GBM. In this review, we intend to highlight these recent advances and the potential future implications of this rapidly evolving field, which should facilitate a better understanding of GBM biology. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://pure.au.dk/portal/da/publications/threedimensional-culture-models-to-study-glioblastoma--current-trends-and-future-perspectivesTest(5c38f0a7-2684-4deb-9e0e-974a82b362a7).html |
| DOI: | 10.1016/j.coph.2021.08.019 |
| الإتاحة: | https://doi.org/10.1016/j.coph.2021.08.019Test https://pure.au.dk/portal/da/publications/threedimensional-culture-models-to-study-glioblastoma--current-trends-and-future-perspectivesTest(5c38f0a7-2684-4deb-9e0e-974a82b362a7).html https://pure.au.dk/ws/files/273706731/1_s2.0_S1471489221001405_main.pdfTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.6150C23 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.coph.2021.08.019 |
|---|