دورية أكاديمية
Nutrigenomic effect of hydroxytyrosol in vascular endothelial cells: A transcriptomic profile analysis
| العنوان: | Nutrigenomic effect of hydroxytyrosol in vascular endothelial cells: A transcriptomic profile analysis |
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| المؤلفون: | Carluccio M. A., Martinelli R., Massaro M., Calabriso N., Scoditti E., Maffia M., Verri T., Gatta V., De Caterina R. |
| المساهمون: | Carluccio, M. A., Martinelli, R., Massaro, M., Calabriso, N., Scoditti, E., Maffia, M., Verri, T., Gatta, V., De Caterina, R. |
| سنة النشر: | 2021 |
| المجموعة: | ARUd'A - Archivio Istituzionale della ricerca dell'università Chieti-Pescara (IRIS) |
| مصطلحات موضوعية: | Endothelial cell, Endothelial dysfunction, Gene expression, Hydroxytyrosol, Microarray analysi, Nutrigenomic, Olive oil polyphenol, Transcriptomic, Down-Regulation, Gene Ontology, Human Umbilical Vein Endothelial Cell, Human, NF-kappa B, Phenylethyl Alcohol, Reproducibility of Result, Signal Transduction, Unfolded Protein Response, Up-Regulation, Gene Expression Profiling, Nutrigenomics |
| الوصف: | Hydroxytyrosol (HT), a peculiar olive and olive oil phenolic antioxidant, plays a significant role in the endothelial and cardiovascular protection associated with olive oil consumption. However, studies examining the effects of HT on the whole-genome expression of endothelial cells, which are prominent targets for vasculo-protective effects of olive oil polyphenols, have been lacking. This study aims to comprehensively evaluate the genomic effects exerted by HT, at the transcriptional level, in endothelial cells under resting or proinflammatory conditions. Human umbilical vein endothelial cells (HUVECs) were treated with 10 μmol/L HT for 1 h and then stimulated with 5 ng/mL interleukin (IL)-1β for 3 h. Total RNA was extracted, and gene expression profile assessed with microarray analysis. Functional enrichment analysis and pathway analysis were performed by Ingenuity Pathways Analysis. Microarray data were validated by qRT-PCR. Fixing a significance threshold at 1.5-fold change, HT affected the expression of 708 and 599 genes, respectively, in HUVECs under resting and IL-1β-stimulated conditions; among these, 190 were common to both conditions. Unfolded protein response (UPR) and endoplasmic reticulum stress resulted from the two top canonical pathways common between HT and HT-IL-1β affected genes. IL-17F/A signaling was found in the top canonical pathways of HT modified genes under resting unstimulated conditions, whereas cardiac hypertrophy signaling was identified among the pathways affected by HT-IL-1β. The transcriptomic analysis allowed pinpointing immunological, inflammatory, proliferative, and metabolic-related pathways as the most affected by HT in endothelial cells. It also revealed previously unsuspected genes and related gene pathways affected by HT, thus broadening our knowledge of its biological properties. The unbiased identification of novel genes regulated by HT improves our understanding of mechanisms by which olive oil prevents or attenuates inflammatory diseases and identifies new genes to ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | ELETTRONICO |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/34836245; info:eu-repo/semantics/altIdentifier/wos/WOS:000725774300001; volume:13; issue:11; firstpage:1; lastpage:24; numberofpages:24; journal:NUTRIENTS; http://hdl.handle.net/11564/769690Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85118684175; https://www.mdpi.com/2072-6643/13/11/3990Test |
| DOI: | 10.3390/nu13113990 |
| الإتاحة: | https://doi.org/10.3390/nu13113990Test http://hdl.handle.net/11564/769690Test https://www.mdpi.com/2072-6643/13/11/3990Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.61462936 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/nu13113990 |
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