دورية أكاديمية
Evaluation of the biological tolerability of the starch-based medical device 4DryField® PH in vitro and in vivo a rat model
| العنوان: | Evaluation of the biological tolerability of the starch-based medical device 4DryField® PH in vitro and in vivo a rat model |
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| المؤلفون: | Poehnert, Daniel, Abbas, Mahmoud, Maegel, Lavinia, Sambale, Franziska, Lavrentieva, Antonina, Kreipe, Hans-Heinrich, Klempnauer, Jürgen, Winny, Markus |
| المصدر: | Journal of Biomaterials Applications 30 (2015), Nr. 4 |
| بيانات النشر: | SAGE Publications Ltd. |
| سنة النشر: | 2015 |
| المجموعة: | Institutional Repository of Leibniz Universität Hannover |
| مصطلحات موضوعية: | 4DryField® PH, adhesion prevention agent, biocompatibility, cytotoxicity, haemostatic agent, polysaccharide, rat model, Adhesion, Animals, Biomedical equipment, Cell culture, Cells, Cytology, Polysaccharides, Tumors, Abdominal injury, Adhesion preventions, Cytotoxic effects, Foreign body reactions, In-vitro testing, Medical Devices, Mononuclear cells, Rats, animal cell, animal experiment, animal model, animal tissue, Article, cell growth, cell viability |
| الوصف: | Purpose To evaluate in vitro cytotoxicity/biocompatibility as well as in vivo tolerability of the novel polysaccharide 4DryField® PH, certified for haemostasis and adhesion prevention. Methods In vitro cytotoxicity/viability testing according to ISO EN 10,993 using murine and human tumour cell lines incubated with 4DryField® PH (PlantTec Medical GmbH). Using a rat model the impact of 4DryField® PH on animals viability and in vivo effects were macro- and micropathologically assessed. Results In vitro testing revealed no cytotoxic effect of 4DryField® PH nor enhancement of viability to tumour cell lines. In vivo viability of rats was unimpaired by 4DryField® PH. Bodyweight loss in animals with abdominal injury plus treatment with 4DryField® PH was in the range of controls and less than in injured rats without treatment. At day 7 after surgery no formation of adhesions, neither macroscopic nor histological remnants nor signs of foreign body reaction were present in animals without injury. In animals with peritoneal injury and 4DryField® PH application, histopathological observation revealed minor residuals of polysaccharide in the depth of wound cavity embedded in a thickened subperitoneal layer; however, with a suggested intact neoperitoneum. The presence of mononuclear cells surrounding polysaccharide particles in varying states of degradation was observable as well. Conclusion 4DryField® PH is not cytotoxic and does not enhance viability of tumour cell lines. High dose of 4DryField® PH of 1.09 g/kg bodyweight is well tolerated and reduces weight loss in animals with peritoneal injury. The biocompatibility of 4DryField® PH can be rated as being excellent. © SAGE Publications. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0885-3282 |
| العلاقة: | http://dx.doi.org/10.15488/2306Test; http://www.repo.uni-hannover.de/handle/123456789/2332Test |
| DOI: | 10.15488/2306 |
| الإتاحة: | https://doi.org/10.15488/2306Test https://doi.org/10.1177/0885328215592257Test http://www.repo.uni-hannover.de/handle/123456789/2332Test |
| حقوق: | Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich. ; frei zugänglich |
| رقم الانضمام: | edsbas.60429F0C |
| قاعدة البيانات: | BASE |
| تدمد: | 08853282 |
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| DOI: | 10.15488/2306 |