دورية أكاديمية

Preterm Intraventricular Hemorrhage-Induced Inflammatory Response in Human Choroid Plexus Epithelial Cells

التفاصيل البيبلوغرافية
العنوان: Preterm Intraventricular Hemorrhage-Induced Inflammatory Response in Human Choroid Plexus Epithelial Cells
المؤلفون: Zsolt Fejes, Marianna Pócsi, Jun Takai, Judit Erdei, Andrea Tóth, Enikő Balogh, Ágnes Rusznyák, Ferenc Fenyvesi, Andrea Nagy, János Kappelmayer, Viktória Jeney, Béla Nagy
المصدر: International Journal of Molecular Sciences; Volume 22; Issue 16; Pages: 8648
بيانات النشر: Multidisciplinary Digital Publishing Institute
سنة النشر: 2021
المجموعة: MDPI Open Access Publishing
مصطلحات موضوعية: cerebrospinal fluid, choroid plexus epithelial cell, heme, inflammation, intraventricular hemorrhage, microRNA, oxidized hemoglobin
جغرافية الموضوع: agris
الوصف: Following an intraventricular hemorrhage (IVH), red blood cell lysis and hemoglobin (Hb) oxidation with the release of heme can cause sterile neuroinflammation. In this study, we measured Hb derivates and cellular adhesion molecules ICAM-1 and VCAM-1 with cell-free miRNAs in cerebrospinal fluid (CSF) samples obtained from Grade-III and Grade-IV preterm IVH infants (IVH-III and IVH-IV, respectively) at multiple time points between days 0–60 after the onset of IVH. Furthermore, human choroid plexus epithelial cells (HCPEpiCs) were incubated with IVH and non-IVH CSF (10 v/v %) for 24 h in vitro to investigate the IVH-induced inflammatory response that was investigated via: (i) HMOX1, IL8, VCAM1, and ICAM1 mRNAs as well as miR-155, miR-223, and miR-181b levels by RT-qPCR; (ii) nuclear translocation of the NF-κB p65 subunit by fluorescence microscopy; and (iii) reactive oxygen species (ROS) measurement. We found a time-dependent alteration of heme, IL-8, and adhesion molecules which revealed a prolonged elevation in IVH-IV vs. IVH-III with higher miR-155 and miR-181b expression at days 41–60. Exposure of HCPEpiCs to IVH CSF samples induced HMOX1, IL8, and ICAM1 mRNA levels along with increased ROS production via the NF-κB pathway activation but without cell death, as confirmed by the cell viability assay. Additionally, the enhanced intracellular miR-155 level was accompanied by lower miR-223 and miR-181b expression in HCPEpiCs after CSF treatment. Overall, choroid plexus epithelial cells exhibit an abnormal cell phenotype after interaction with pro-inflammatory CSF of IVH origin which may contribute to the development of later clinical complications in preterm IVH.
نوع الوثيقة: text
وصف الملف: application/pdf
اللغة: English
العلاقة: Molecular Biophysics; https://dx.doi.org/10.3390/ijms22168648Test
DOI: 10.3390/ijms22168648
الإتاحة: https://doi.org/10.3390/ijms22168648Test
حقوق: https://creativecommons.org/licenses/by/4.0Test/
رقم الانضمام: edsbas.60232CDE
قاعدة البيانات: BASE