التفاصيل البيبلوغرافية
| العنوان: |
Overexpression of calreticulin increases the Ca 2� capacity of rapidly exchanging Ca 2� stores and reveals aspects of their lumenal microenvironment and function |
| المؤلفون: |
Carlo Bastianutto, Emilio Clementi, Franca Codazzi, Paola Podini, Francesca De Giorgi, Rosario Rizzuto, Jacopo Meldolesi, Tullio Pozzan |
| المساهمون: |
The Pennsylvania State University CiteSeerX Archives |
| المصدر: |
ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/3c/df/J_Cell_Biol_1995_Aug_2_130(4)_847-855.tar.gz |
| سنة النشر: |
1995 |
| المجموعة: |
CiteSeerX |
| الوصف: |
A molecularly tagged form of calreticulin (CR), a low affinity-high capacity Ca 2+ binding protein that resides in the ER lumen, was transiently transfected into HeLa cells to specifically modify the Ca 2÷ buffering capacity of the intracellular Ca 2 ÷ stores. Fluorescence and confocal microscope immunocytochemistry revealed the tagged protein to be expressed by over 40 % of the cells and to overlap in its distribution the endogenous CR yielding a delicate cytoplasmic network, i.e., the typical pattern of ER. In contrast, no signal was observed associated with the plasmalemma (marked by ConA) and within the nucleus. One- and two-dimensional Western blots revealed the transfected to exceed the endogenous CR of ~3.5-fold and to maintain its Ca 2+ binding ability, whereas the expression |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/zip |
| اللغة: |
English |
| العلاقة: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.283.214Test |
| حقوق: |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: |
edsbas.6007121D |
| قاعدة البيانات: |
BASE |
