التفاصيل البيبلوغرافية
| العنوان: |
Single-Molecule Localization Microscopy of Subcellular Protein Distribution in Neurons |
| المؤلفون: |
Willems, Jelmer, Westra, Manon, MacGillavry, Harold D |
| المساهمون: |
Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Heit, Bryan |
| سنة النشر: |
2022 |
| مصطلحات موضوعية: |
Neuron, Photoactivated localization microscopy, Single-molecule localization microscopy, Stochastic optical reconstruction microscopy, Superresolution microscopy, Synapse, Taverne, Genetics, Molecular Biology |
| الوصف: |
Over the past years several forms of superresolution fluorescence microscopy have been developed that offer the possibility to study cellular structures and protein distribution at a resolution well below the diffraction limit of conventional fluorescence microscopy (<200 nm). A particularly powerful superresolution technique is single-molecule localization microscopy (SMLM). SMLM enables the quantitative investigation of subcellular protein distribution at a spatial resolution up to tenfold higher than conventional imaging, even in live cells. Not surprisingly, SMLM has therefore been used in many applications in biology, including neuroscience. This chapter provides a step-by-step SMLM protocol to visualize the nanoscale organization of endogenous proteins in dissociated neurons but can be extended to image other adherent cultured cells. We outline a number of methods to visualize endogenous proteins in neurons for live-cell and fixed application, including immunolabeling, the use of intrabodies for live-cell SMLM, and endogenous tagging using CRISPR/Cas9. |
| نوع الوثيقة: |
book part |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| تدمد: |
1064-3745 |
| العلاقة: |
https://dspace.library.uu.nl/handle/1874/424052Test |
| الإتاحة: |
https://dspace.library.uu.nl/handle/1874/424052Test |
| حقوق: |
info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: |
edsbas.5F923D3C |
| قاعدة البيانات: |
BASE |
