كتاب
2D NMR study of lignin modification during pretreatment of Eucalyptus wood feedstock with a fungal laccase and a phenolic mediator
| العنوان: | 2D NMR study of lignin modification during pretreatment of Eucalyptus wood feedstock with a fungal laccase and a phenolic mediator |
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| المؤلفون: | Rico Campos, Alejandro, Rencoret, Jorge, Río Andrade, José Carlos del, Martínez, Ángel T., Gutiérrez Suárez, Ana |
| بيانات النشر: | CSIC - Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS) |
| سنة النشر: | 2014 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Eucalyptus wood, Delignification, Enzymatic pretreatment, Laccases, Lignin, 2D NMR |
| الوصف: | The delignification of eucalypt feedstock during enzymatic pretreatment with the laccase from Myceliophthora thermophila, in conjunction with methyl syringate as mediator, when applied in a multistage sequence consisting of successive enzymatic and alkaline extraction stages, directly on the ground lignocellulosic material was thoroughly studied by two-dimensional nuclear magnetic resonance (2D NMR) of the whole wood (at the gel stage) after each step of the sequence. The 2D NMR analyses of eucalypt feedstock after the enzymatic pretreatment, revealed the removal of guaiacyl and syringyl lignin units (with preferential removal of the former) and aliphatic (mainly beta-O-4'-linked) side-chains of lignin without a substantial change in polysaccharide crosssignals. However, the most noticeable modification was the formation of Calpha-oxidized syringyl lignin units in the enzymatic steps that were partially removed in the alkaline extraction stages. This study revealed that the first cycle of pretreatment of eucalypt with M. thermophila laccase and methyl syringate did not produce any delignification effect and consequently did not increase the saccharification yields. However, at the end of the whole sequence (4 cycles), a high delignification (ca. 50%) of eucalypt feedstock and increases (ca. 40%) in glucose yield after enzymatic hydrolysis was attained with this laccase-mediator pretreatment. ; This study was funded by the INDOX (KBBE-2013-7-613549) EU-project and the LIGNOCELL (AGL2011-25379) Spanish MICINN project (co-financed by FEDER funds). J. Rencoret acknowledges the CSIC JAE-Doc contract of the program “Junta para la Ampliación de Estudios” co-financed by the European Social Fund (ESF). H. Lund and L. Kalum from Novozymes (Bagsvaerd, Denmark) are acknowledged for the cellulases and the M. thermophila laccase and J. Romero (ENCE, Spain) for the eucalypt wood samples. Manuel Angulo (CITIUS, University of Seville) is acknowledged for providing technical assistance in the NMR analyses. ; Peer reviewed |
| نوع الوثيقة: | book part |
| اللغة: | English |
| ردمك: | 978-84-616-9842-4 84-616-9842-8 |
| العلاقة: | #PLACEHOLDER_PARENT_METADATA_VALUE#; KBBE-2013-7-613549; Sí; Proceedings of the 13th European Workshop on Lignocellulosics and Pulp, pp. 711-714 (2014); http://hdl.handle.net/10261/98720Test |
| الإتاحة: | http://hdl.handle.net/10261/98720Test |
| حقوق: | open |
| رقم الانضمام: | edsbas.5EFF6BE8 |
| قاعدة البيانات: | BASE |
| ردمك: | 9788461698424 8461698428 |
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