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Regulation by reactive oxygen species of interleukin-1beta, nitric oxide and prostaglandin E(2) production by human chondrocytes.

التفاصيل البيبلوغرافية
العنوان: Regulation by reactive oxygen species of interleukin-1beta, nitric oxide and prostaglandin E(2) production by human chondrocytes.
المؤلفون: Mathy, Marianne, Deby, Ginette, Reginster, Jean-Yves, Ayache, N., Pujol, J*-P, Henrotin, Yves
المصدر: Osteoarthritis and Cartilage, 10 (7), 547-55 (2002)
بيانات النشر: W.B. Saunders
سنة النشر: 2002
المجموعة: University of Liège: ORBi (Open Repository and Bibliography)
مصطلحات موضوعية: Anti-Inflammatory Agents, Non-Steroidal/pharmacology, Antioxidants/pharmacology, Cartilage, Articular/drug effects/metabolism/pathology, Cell Culture Techniques, Chondrocytes/drug effects/metabolism, Cyclooxygenase 2, Dinoprostone/biosynthesis, Female, Gene Expression Regulation/drug effects, Humans, Interleukin-1/biosynthesis/genetics, Isoenzymes/biosynthesis/genetics, Male, Membrane Proteins, Middle Aged, Nitric Oxide/biosynthesis/physiology, Nitric Oxide Synthase/biosynthesis/genetics, Nitric Oxide Synthase Type II, Osteoarthritis, Knee/metabolism, Prostaglandin-Endoperoxide Synthases/biosynthesis/genetics, RNA, Messenger/genetics, Reactive Oxygen Species/metabolism, Human health sciences, General & internal medicine, Sciences de la santé humaine, Médecine générale & interne
الوصف: peer reviewed ; OBJECTIVES: To determine the effects of two drugs, N-monomethyl-L-arginine (L-NMMA) and N-acetylcysteine (NAC), on interleukin-1beta (IL-1beta), nitric oxide (NO) and prostaglandin E(2) (PGE(2)) production by human chondrocytes. The effect of aceclofenac (ACECLO), a non-steroidal antiinflammatory drug (NSAID), was also examined. METHODS: Human chondrocytes were enzymatically isolated from osteoarthritic knee cartilage and then maintained in culture in suspension for 48h in the absence or in the presence of lipopolysaccharide (LPS) (10 microg/ml), L-NMMA (0.5mM), NAC (1mM) or ACECLO (6.10(-6)M). IL-1beta and PGE(2) productions were quantified by specific immunoassays. Nitrite was measured in the culture supernatants by a spectrophotometric method based upon the Griess reaction. Cyclooxygenase-2 (COX-2), inducible NO synthase (iNOS) and IL-1beta gene expressions were quantified by transcription of mRNA followed by real time and quantitative polymerase chain reaction. COX-2 protein expression was analysed by Western blot. RESULTS: LPS markedly increased the expression of IL-1beta, iNOS and COX-2 genes. In parallel, NO(2) and PGE(2) amounts found in the culture supernatants were significantly enhanced whereas IL-1beta was immunologically undetectable. The addition of L-NMMA (0.5mM) fully blocked LPS-induced NO production but greatly increased PGE(2) production, suggesting a negative effect of NO on PGE(2) synthesis. Inversely, NO production was stimulated by NAC while PGE(2) production was not affected. Interestingly, NAC increased the IL-1beta and iNOS mRNA levels but did not significantly modify COX-2 mRNA expression. L-NMMA did not significantly affect the expression of IL-1beta, iNOS and COX-2. The amount of COX-2 protein did not change in the presence of the antioxidants. Finally, ACECLO fully blocked the production of PGE(2) by chondrocytes without affecting the levels of COX-2 mRNA. CONCLUSIONS: The stimulation of IL-1beta, NO and PGE(2) production by LPS is differentially controlled by ...
نوع الوثيقة: article in journal/newspaper
اللغة: English
تدمد: 1063-4584
1522-9653
العلاقة: urn:issn:1063-4584; urn:issn:1522-9653; https://orbi.uliege.be/handle/2268/10306Test; info:hdl:2268/10306; scopus-id:2-s2.0-0036638431; info:pmid:12127835
DOI: 10.1053/joca.2002.0789
الإتاحة: https://doi.org/10.1053/joca.2002.0789Test
https://orbi.uliege.be/handle/2268/10306Test
حقوق: restricted access ; http://purl.org/coar/access_right/c_16ecTest ; info:eu-repo/semantics/restrictedAccess
رقم الانضمام: edsbas.5E1BBD2A
قاعدة البيانات: BASE
الوصف
تدمد:10634584
15229653
DOI:10.1053/joca.2002.0789