دورية أكاديمية
Light-Induced Production of An Antibody Fragment and Malaria Vaccine Antigen from Chlamydomonas reinhardtii
| العنوان: | Light-Induced Production of An Antibody Fragment and Malaria Vaccine Antigen from Chlamydomonas reinhardtii |
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| المؤلفون: | Neera Munjal, Andrea Juliana Garzon-Sanabria, Katelyn Wilson Quinones, James Gregory, Zivko L. Nikolov |
| المصدر: | Processes, Vol 2, Iss 3, Pp 625-638 (2014) |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2014 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | single-chain antibody fragment, vaccine antigen, Pfs25, Chlamydomonas reinhardtii, cultivation, recombinant protein accumulation, light induction, Chemical technology, TP1-1185, Chemistry, QD1-999 |
| الوصف: | The eukaryotic green alga, Chlamydomonas reinhardtii, is a unique expression platform that can efficiently express complex therapeutic proteins. However, demonstrating that therapeutic molecules can be produced in quantifiable levels is essential to establish the potential of the C. reinhardtii expression system. Thus, the objective of this investigation was to determine the process conditions that could maximize C. reinhardtii biomass accumulation and induced-production of the two recombinant proteins, a single chain fragment antibody molecule (αCD22 scFv) and malaria vaccine antigen (Pfs25), produced in the chloroplast of C. reinhardtii. To achieve a higher production of recombinant proteins, cultivation variables of C. reinhardtii, such as mixing, light-induction time and intensity, nutrient depletion and culture age, were investigated and optimized. The optimal light-induction time was 24 h at a light intensity of 300 μmol m−2 s−1. Replacement of the culture media in the late exponential growth with fresh media was beneficial to the accumulation of recombinant proteins. Optimization led to increases in the accumulation of recombinant proteins by six-fold and the recombinant protein fraction in the extracted soluble protein by two-fold. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2227-9717 |
| العلاقة: | http://www.mdpi.com/2227-9717/2/3/625Test; https://doaj.org/toc/2227-9717Test; https://doaj.org/article/b7174ac6e61643108c1c638213b1eb46Test |
| DOI: | 10.3390/pr2030625 |
| الإتاحة: | https://doi.org/10.3390/pr2030625Test https://doaj.org/article/b7174ac6e61643108c1c638213b1eb46Test |
| رقم الانضمام: | edsbas.5D950127 |
| قاعدة البيانات: | BASE |
| تدمد: | 22279717 |
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| DOI: | 10.3390/pr2030625 |