دورية أكاديمية
Oncostatin M Counteracts the Fibrotic Effects of TGF-β1 and IL-4 on Nasal-Polyp-Derived Fibroblasts: A Control of Fibrosis in Chronic Rhinosinusitis with Nasal Polyps?
| العنوان: | Oncostatin M Counteracts the Fibrotic Effects of TGF-β1 and IL-4 on Nasal-Polyp-Derived Fibroblasts: A Control of Fibrosis in Chronic Rhinosinusitis with Nasal Polyps? |
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| المؤلفون: | Carsuzaa, Florent, Béquignon, Émilie, Bainaud, Matthieu, Jégou, Jean-François, Dufour, Xavier, Lecron, Jean-Claude, Favot, Laure |
| المساهمون: | Laboratoire Inflammation, Tissus épithéliaux et Cytokines (LITEC Poitiers ), Université de Poitiers = University of Poitiers (UP), Service ORL, Chirurgie Cervico‐Maxillo‐Faciale et Audiophonologie, Centre Hospitalier Universitaire de Poitiers, Service ORL et Chirurgie Cervico‐Faciale, Centre Hospitalier Intercommunal de Créteil, Inserm, UMR-955, F-94000 Créteil, France, Service Immunologie et Inflammation, Centre Hospitalier Universitaire de Poitiers |
| المصدر: | ISSN: 1661-6596. |
| بيانات النشر: | HAL CCSD MDPI |
| سنة النشر: | 2022 |
| المجموعة: | Université de Poitiers: Publications de nos chercheurs.ses (HAL) |
| مصطلحات موضوعية: | [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.IMM]Life Sciences [q-bio]/Immunology, [SDV.MHEP.DERM]Life Sciences [q-bio]/Human health and pathology/Dermatology |
| الوصف: | International audience ; Chronic rhinosinusitis with nasal polyps (CRSwNP) is associated with inflammation and tissue remodeling including myofibroblasts differentiation and extracellular matrix (ECM) deposition mediated by TGF-β1 and IL-4. Oncostatin M (OSM) is a cytokine involved in fibrotic processes in other cellular subtypes. We investigated the mechanisms of action of OSM in the fibrosis process associated with CRSwNP. The expression of IL-4, OSM and TGF-β1 was assessed by RT-qPCR. Primary human cultures of nasal-polyp-derived fibroblasts were established and stimulated by TGF-β1 and/or IL-4 and/or OSM. The expression of ECM components and αSMA was determined by RT-qPCR and Western blot. TGF-β1-Smad3 signaling was investigated by immunofluorescence. TGF-β1, IL-4 and OSM as well as αSMA were overexpressed in nasal polyps when compared to noninflammatory nasal mucosa. In TGF-β1-stimulated nasal-polyp-derived fibroblasts, ECM genes and αSMA gene and protein were overexpressed, as well as αSMA in IL-4-stimulated fibroblasts. OSM counteracted the profibrotic effect of TGF-β1 on ECM components and αSMA. TGF-β1-induced nuclear translocation of Smad3 was completely reversed by OSM. OSM counteracts the profibrotic effect of IL-4 and also TGF-β1, by inhibiting the nuclear translocation of Smad3. We suggest OSM could be an efficient tool to protect against fibrosis in CRSwNP. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-03801056; https://hal.science/hal-03801056Test; https://hal.science/hal-03801056/documentTest; https://hal.science/hal-03801056/file/ijms-23-06308-v3.pdfTest |
| DOI: | 10.3390/ijms23116308 |
| الإتاحة: | https://doi.org/10.3390/ijms23116308Test https://hal.science/hal-03801056Test https://hal.science/hal-03801056/documentTest https://hal.science/hal-03801056/file/ijms-23-06308-v3.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.5D4AFAF9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/ijms23116308 |
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