دورية أكاديمية
Chromatin organization at the nuclear pore favours HIV replication.
| العنوان: | Chromatin organization at the nuclear pore favours HIV replication. |
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| المؤلفون: | Lelek, Mickaël, Casartelli, Nicoletta, Pellin, Danilo, Rizzi, Ermanno, Souque, Philippe, Severgnini, Marco, Di Serio, Clelia, Fricke, Thomas, Diaz-Griffero, Felipe, Zimmer, Christophe, Charneau, Pierre, Di Nunzio, Francesca |
| المساهمون: | Imagerie et Modélisation, Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS), Virus et Immunité, Vita-Salute San Raffaele University and Center for Translational Genomics and Bioinformatics, Institute for Biomedical Technologies (ITB), National Research Council of Italy, Virologie moléculaire et Vaccinologie, Albert Einstein College of Medicine New York, This work was funded by the ANRS (Agence Nationale de Recherche sur le SIDA), the Sidaction, the Pasteur Institute. C.Z. acknowledges support from Institut Pasteur, Région Ile de France (DIM Malinf), Fondation pour la Recherche Médicale (Equipe FRM 2010) and ANRS. E.R. was funded by ‘Futuro in Ricerca’ grant n° RBFR126B8I_003. F.D.G and T.F. were supported by grants NIH R01 AI087390, R21 AI102824 and R56 AI108432 to F.D.G. |
| المصدر: | ISSN: 2041-1723. |
| بيانات النشر: | HAL CCSD Nature Publishing Group |
| سنة النشر: | 2015 |
| المجموعة: | Institut Pasteur: HAL |
| مصطلحات موضوعية: | MESH: Adaptor Proteins, Signal Transducing, MESH: Blotting, Western, MESH: Virus Integration, MESH: Virus Replication, MESH: Chromatin, MESH: Gene Expression Profiling, MESH: HEK293 Cells, MESH: HIV-1, MESH: HeLa Cells, MESH: Humans, MESH: Jurkat Cells, MESH: Luciferases, MESH: Microscopy, Confocal, MESH: Nuclear Pore, MESH: Nuclear Pore Complex Proteins, MESH: Oligonucleotides, MESH: Proto-Oncogene Proteins, MESH: Real-Time Polymerase Chain Reaction, MESH: Reverse Transcriptase Polymerase Chain Reaction, MESH: Transcription Factors, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; The molecular mechanisms that allow HIV to integrate into particular sites of the host genome are poorly understood. Here we tested if the nuclear pore complex (NPC) facilitates the targeting of HIV integration by acting on chromatin topology. We show that the integrity of the nuclear side of the NPC, which is mainly composed of Tpr, is not required for HIV nuclear import, but that Nup153 is essential. Depletion of Tpr markedly reduces HIV infectivity, but not the level of integration. HIV integration sites in Tpr-depleted cells are less associated with marks of active genes, consistent with the state of chromatin proximal to the NPC, as analysed by super-resolution microscopy. LEDGF/p75, which promotes viral integration into active genes, stabilizes Tpr at the nuclear periphery and vice versa. Our data support a model in which HIV nuclear import and integration are concerted steps, and where Tpr maintains a chromatin environment favourable for HIV replication. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/25744187; pasteur-01536149; https://pasteur.hal.science/pasteur-01536149Test; https://pasteur.hal.science/pasteur-01536149/documentTest; https://pasteur.hal.science/pasteur-01536149/file/ncomms7483.pdfTest; PUBMED: 25744187; PUBMEDCENTRAL: PMC4366494 |
| DOI: | 10.1038/ncomms7483 |
| الإتاحة: | https://doi.org/10.1038/ncomms7483Test https://pasteur.hal.science/pasteur-01536149Test https://pasteur.hal.science/pasteur-01536149/documentTest https://pasteur.hal.science/pasteur-01536149/file/ncomms7483.pdfTest |
| حقوق: | http://creativecommons.org/licenses/byTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.5D44F27 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/ncomms7483 |
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