دورية أكاديمية
The nitrone spin trap 5,5-dimethyl-1-pyrroline N-oxide binds to toll-like receptor-2-TIR-BB-loop domain and dampens downstream inflammatory signaling
| العنوان: | The nitrone spin trap 5,5-dimethyl-1-pyrroline N-oxide binds to toll-like receptor-2-TIR-BB-loop domain and dampens downstream inflammatory signaling |
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| المؤلفون: | Muñoz, Marcos, Gutierrez, Lucas, J, Delignat, Sandrine, Russick, Jules, Gomez, Sandra, Lacroix-Desmazes, Sébastien, Enriz, Daniel, R, Ramirez, Dario, C |
| المساهمون: | Centre de Recherche des Cordeliers (CRC), Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École Pratique des Hautes Études (EPHE), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Laboratoire des sciences et techniques de l'information, de la communication et de la connaissance (Lab-STICC), École Nationale d'Ingénieurs de Brest (ENIB)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Institut Mines-Télécom Paris (IMT)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL)-IMT Atlantique (IMT Atlantique), Institut Mines-Télécom Paris (IMT) |
| المصدر: | ISSN: 0925-4439 ; Biochimica et Biophysica Acta - Molecular Basis of Disease ; https://hal.science/hal-02328201Test ; Biochimica et Biophysica Acta - Molecular Basis of Disease, 2019, ⟨10.1016/j.bbadis.2019.01.005⟩. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | [SDV.IMM]Life Sciences [q-bio]/Immunology |
| الوصف: | International audience ; The nitrone spin trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO) dampens endotoxin-induced and TLR4-driven priming of macrophages, but the mechanism remains unknown. The available information suggests a direct binding of DMPO to the TIR domain, which is shared between TLRs. However, TLR2-TIR domain is the only TLR that have been crystallized. Our in silico data show that DMPO binds to four specific residues in the BB-loop within the TLR2-TIR domain. Our functional analysis using hTLR2.6-expressing HEKs cells showed that DMPO can block zymosan-triggered-TLR2-mediated NF-kb activation. However, DMPO did not affect the overall TLR2-MyD88 protein-protein interaction. DMPO binds to the BB-loop in the TIR-domain and dampens downstream signaling without affecting the overall TIR-MyD88 interaction. These data encourage the use of DMPO-derivatives as potential mechanism-based inhibitors of TLR-triggered inflammation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/30684639; hal-02328201; https://hal.science/hal-02328201Test; https://hal.science/hal-02328201/documentTest; https://hal.science/hal-02328201/file/2019%20Munoz%20BBA%20TLR2%20DMPO.pdfTest; PUBMED: 30684639 |
| DOI: | 10.1016/j.bbadis.2019.01.005 |
| الإتاحة: | https://doi.org/10.1016/j.bbadis.2019.01.005Test https://hal.science/hal-02328201Test https://hal.science/hal-02328201/documentTest https://hal.science/hal-02328201/file/2019%20Munoz%20BBA%20TLR2%20DMPO.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.5CE921E5 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.bbadis.2019.01.005 |
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