دورية أكاديمية
Impact of silica nanoparticle surface chemistry on protein corona formation and consequential interactions with biological cells
| العنوان: | Impact of silica nanoparticle surface chemistry on protein corona formation and consequential interactions with biological cells |
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| المؤلفون: | Kurtz-Chalot, Andréa, Villiers, Christian, L., Pourchez, Jérémie, Boudard, Delphine, Martini, Matteo, Marche, Patrice, Cottier, Michèle, Forest, Valérie |
| المساهمون: | Santé Ingénierie Biologie Saint-Etienne (SAINBIOSE), Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire CHU Grenoble (CHUGA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes 2016-2019 (UGA 2016-2019 ), Institut Lumière Matière Villeurbanne (ILM), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 0928-4931. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2017 |
| المجموعة: | Université Jean Monnet – Saint-Etienne: HAL |
| مصطلحات موضوعية: | [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering |
| الوصف: | International audience ; Nanoparticles (NP) physico-chemical features greatly influence NP/cell interactions. NP surface functionalization is often used to improve NP biocompatibility or to enhance cellular uptake. But in biological media, the formation of a protein corona adds a level of complexity. The aim of this study was to investigate in vitro the influence of NP surface functionalization on their cellular uptake and the biological response induced. 50nm fluorescent silica NP were functionalized either with amine or carboxylic groups, in presence or in absence of polyethylene glycol (PEG). NP were incubated with macrophages, cellular uptake and cellular response were assessed in terms of cytotoxicity, pro-inflammatory response and oxidative stress. The NP protein corona was also characterized by protein mass spectroscopy. Results showed that NP uptake was enhanced in absence of PEG, while NP adsorption at the cell membrane was fostered by an initial positively charged NP surface. NP toxicity was not correlated with NP uptake. NP surface functionalization also influenced the formation of the protein corona as the profile of protein binding differed among the NP types. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-01680498; https://hal.science/hal-01680498Test; https://hal.science/hal-01680498/documentTest; https://hal.science/hal-01680498/file/2017_HAL_Kurtz-Chalot_MSEC.pdfTest |
| DOI: | 10.1016/j.msec.2017.02.028 |
| الإتاحة: | https://doi.org/10.1016/j.msec.2017.02.028Test https://hal.science/hal-01680498Test https://hal.science/hal-01680498/documentTest https://hal.science/hal-01680498/file/2017_HAL_Kurtz-Chalot_MSEC.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.5CE91F2A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.msec.2017.02.028 |
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