التفاصيل البيبلوغرافية
| العنوان: |
Combinatorial Assembly of Modular Glucosides via Carboxylesterases Regulates C. elegans Starvation Survival |
| المؤلفون: |
Chester J. J. Wrobel (11356554), Jingfang Yu (1428361), Pedro R. Rodrigues (9520246), Andreas H. Ludewig (3916223), Brian J. Curtis (9536359), Sarah M. Cohen (11356557), Bennett W. Fox (9520243), Michael P. O’Donnell (4836129), Paul W. Sternberg (5790), Frank C. Schroeder (159898) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Cell Biology, Genetics, Physiology, Biological Sciences not elsewhere classified, related nematode species, providing first insights, mechanisms connecting nutrition, diverse glucose scaffolds, cas9 genome editing, premier model systems, >- deletion results, specific 2 -<, 150 different mogls, carboxylesterase homologue cel, model organism, closest homologue, >- acylation, wide variety, strongly induced, starvation survival, starvation conditions, recently discovered, mogl biosynthesis, lipid metabolism, early death, comparative metabolomics, combining crispr, combinatorial assembly, building blocks |
| الوصف: |
The recently discovered mo dular gl ucosides (MOGLs) form a large metabolite library derived from combinatorial assembly of moieties from amino acid, neurotransmitter, and lipid metabolism in the model organism C. elegans . Combining CRISPR-Cas9 genome editing, comparative metabolomics, and synthesis, we show that the carboxylesterase homologue Cel-CEST-1.2 is responsible for specific 2- O -acylation of diverse glucose scaffolds with a wide variety of building blocks, resulting in more than 150 different MOGLs. We further show that this biosynthetic role is conserved for the closest homologue of Cel-CEST-1.2 in the related nematode species C. briggsae , Cbr-CEST-2. Expression of Cel-cest-1.2 and MOGL biosynthesis are strongly induced by starvation conditions in C. elegans , one of the premier model systems for mechanisms connecting nutrition and physiology. Cel-cest-1.2 -deletion results in early death of adult animals under starvation conditions, providing first insights into the biological functions of MOGLs. |
| نوع الوثيقة: |
dataset |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/dataset/Combinatorial_Assembly_of_Modular_Glucosides_via_Carboxylesterases_Regulates_i_C_elegans_i_Starvation_Survival/16543482Test |
| DOI: |
10.1021/jacs.1c05908.s003 |
| الإتاحة: |
https://doi.org/10.1021/jacs.1c05908.s003Test |
| حقوق: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.59E66C19 |
| قاعدة البيانات: |
BASE |
