دورية أكاديمية
Host Responses to Intestinal Microbial Antigens in Gluten-Sensitive Mice
| العنوان: | Host Responses to Intestinal Microbial Antigens in Gluten-Sensitive Mice |
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| المؤلفون: | Natividad, Jane M., Huang, Xianxi, Slack, Emma, id_orcid:0 000-0002-2473-1145, Jury, Jennifer, Sanz, Yolanda, David, Chella, Denou, Emmanuel, Yang, Pinchang, Murray, Joseph, McCoy, Kathy D., Verdu, Elena F. |
| المصدر: | PLoS ONE, 4 (7) |
| بيانات النشر: | PLOS |
| سنة النشر: | 2009 |
| المجموعة: | ETH Zürich Research Collection |
| الوصف: | Background and Aims Excessive uptake of commensal bacterial antigens through a permeable intestinal barrier may influence host responses to specific antigen in a genetically predisposed host. The aim of this study was to investigate whether intestinal barrier dysfunction induced by indomethacin treatment affects the host response to intestinal microbiota in gluten-sensitized HLA-DQ8/HCD4 mice. Methodology/Principal Findings HLA-DQ8/HCD4 mice were sensitized with gluten, and gavaged with indomethacin plus gluten. Intestinal permeability was assessed by Ussing chamber; epithelial cell (EC) ultra-structure by electron microscopy; RNA expression of genes coding for junctional proteins by Q-real-time PCR; immune response by in-vitro antigen-specific T-cell proliferation and cytokine analysis by cytometric bead array; intestinal microbiota by fluorescence in situ hybridization and analysis of systemic antibodies against intestinal microbiota by surface staining of live bacteria with serum followed by FACS analysis. Indomethacin led to a more pronounced increase in intestinal permeability in gluten-sensitized mice. These changes were accompanied by severe EC damage, decreased E-cadherin RNA level, elevated IFN-γ in splenocyte culture supernatant, and production of significant IgM antibody against intestinal microbiota. Conclusion Indomethacin potentiates barrier dysfunction and EC injury induced by gluten, affects systemic IFN-γ production and the host response to intestinal microbiota antigens in HLA-DQ8/HCD4 mice. The results suggest that environmental factors that alter the intestinal barrier may predispose individuals to an increased susceptibility to gluten through a bystander immune activation to intestinal microbiota. ; ISSN:1932-6203 |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/application/pdf |
| اللغة: | English |
| العلاقة: | http://hdl.handle.net/20.500.11850/113238Test |
| DOI: | 10.3929/ethz-b-000113238 |
| الإتاحة: | https://doi.org/20.500.11850/113238Test https://doi.org/10.3929/ethz-b-000113238Test https://doi.org/10.1371/journal.pone.0006472Test https://hdl.handle.net/20.500.11850/113238Test |
| حقوق: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/3.0Test/ ; Creative Commons Attribution 3.0 Unported |
| رقم الانضمام: | edsbas.59D08D14 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3929/ethz-b-000113238 |
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