دورية أكاديمية
Down-regulation of {beta}1,4-galactosyltransferase V is a critical part of etoposide-induced apoptotic process and could be mediated by decreasing Sp1 levels in human glioma cells
| العنوان: | Down-regulation of {beta}1,4-galactosyltransferase V is a critical part of etoposide-induced apoptotic process and could be mediated by decreasing Sp1 levels in human glioma cells |
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| المؤلفون: | Jiang, Jianhai, Shen, Jialin, Wei, Yuanyan, Wu, Tao, Chen, Xiaoning, Zong, Hongliang, Zhang, Si, Sun, Maoyun, Xie, Jianhui, Kong, Xiangfei, Yang, Yanzhong, Shen, Aiguo, Wang, Hanzhou, Gu, Jianxin |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2006 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | Article |
| الوصف: | β 1,4-Galactosyltransferase V ( β 1,4GalT V; EC 2.4.1.38) is considered to be very important in glioma for expressing transformation-related highly branched N -glycans. Recently, we have characterized β 1,4GalT V as a positive growth regulator in several glioma cell lines. However, the role of β 1,4GalT V in glioma therapy has not been clearly reported. In this study, interfering with the expression of β 1,4GalT V by its antisense cDNA in SHG44 human glioma cells markedly promoted apoptosis induced by etoposide and activation of caspases as well as processing of Bid and expression of Bax and Bak. Conversely, the ectopic expression of β 1,4GalT V attenuated the apoptotic effect of etoposide on SHG44 cells. In addition, both the β 1,4GalT V transcription and the binding of total or membrane glycoprotein with RCA-I were partially reduced in etoposide-treated SHG44 cells, correlated well with a decreased level of Sp1 which has been identified as an activator of β 1,4GalT V transcription. Collectively, our results suggest that down-regulation of β 1,4GalT V expression plays an important role in etoposide-induced apoptosis and could be mediated by a decreasing level of Sp1 in SHG44 cells, indicating that inhibitors of β 1,4GalT V may enhance the therapeutic efficiency of etoposide for malignant glioma. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| العلاقة: | http://glycob.oxfordjournals.org/cgi/content/short/cwl027v1Test; http://dx.doi.org/10.1093/glycob/cwl027Test |
| DOI: | 10.1093/glycob/cwl027 |
| الإتاحة: | https://doi.org/10.1093/glycob/cwl027Test http://glycob.oxfordjournals.org/cgi/content/short/cwl027v1Test |
| حقوق: | Copyright (C) 2006, Society for Glycobiology |
| رقم الانضمام: | edsbas.5933E583 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/glycob/cwl027 |
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