دورية أكاديمية
Exploring the potential role of four Rhizophagus irregularis nuclear effectors: opportunities and technical limitations
| العنوان: | Exploring the potential role of four Rhizophagus irregularis nuclear effectors: opportunities and technical limitations |
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| المؤلفون: | Aparicio Chacón, María Victoria, Hernández Luelmo, Sofía, Devlieghere, Viktor, Robichez, Louis, Leroy, Toon, Stuer, Naomi, De Keyser, Annick, Ceulemans, Evi, Goossens, Alain, Goormachtig, Sofie, Van Dingenen, Judith |
| المصدر: | Frontiers in Plant Science ; ISSN: 1664-462X |
| بيانات النشر: | Frontiers Media SA |
| سنة النشر: | 2024 |
| المجموعة: | Ghent University Academic Bibliography |
| مصطلحات موضوعية: | Biology and Life Sciences, Rhizophagus irregularis, arbuscular mycorrhizal symbiosis, effectors, mycorrhization, nuclear effector proteins, plant growth, protein-protein interaction, transcriptional responses |
| الوصف: | Arbuscular mycorrhizal fungi (AMF) are obligate symbionts that interact with the roots of most land plants. The genome of the AMF model species Rhizophagus irregularis contains hundreds of predicted small effector proteins that are secreted extracellularly but also into the plant cells to suppress plant immunity and modify plant physiology to establish a niche for growth. Here, we investigated the role of four nuclear-localized putative effectors, i.e., GLOIN707, GLOIN781, GLOIN261, and RiSP749, in mycorrhization and plant growth. We initially intended to execute the functional studies in Solanum lycopersicum, a host plant of economic interest not previously used for AMF effector biology, but extended our studies to the model host Medicago truncatula as well as the non-host Arabidopsis thaliana because of the technical advantages of working with these models. Furthermore, for three effectors, the implementation of reverse genetic tools, yeast two-hybrid screening and whole-genome transcriptome analysis revealed potential host plant nuclear targets and the downstream triggered transcriptional responses. We identified and validated a host protein interactors participating in mycorrhization in the host.S. lycopersicum and demonstrated by transcriptomics the effectors possible involvement in different molecular processes, i.e., the regulation of DNA replication, methylglyoxal detoxification, and RNA splicing. We conclude that R. irregularis nuclear-localized effector proteins may act on different pathways to modulate symbiosis and plant physiology and discuss the pros and cons of the tools used. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | https://biblio.ugent.be/publication/01HYEC2KS83W7BZ75VKS04GTPHTest; http://hdl.handle.net/1854/LU-01HYEC2KS83W7BZ75VKS04GTPHTest; http://doi.org/10.3389/fpls.2024.1384496Test; https://biblio.ugent.be/publication/01HYEC2KS83W7BZ75VKS04GTPH/file/01HYECK021FCE46HC5SVS91RW1Test |
| DOI: | 10.3389/fpls.2024.1384496 |
| الإتاحة: | https://doi.org/10.3389/fpls.2024.1384496Test https://biblio.ugent.be/publication/01HYEC2KS83W7BZ75VKS04GTPHTest http://hdl.handle.net/1854/LU-01HYEC2KS83W7BZ75VKS04GTPHTest https://biblio.ugent.be/publication/01HYEC2KS83W7BZ75VKS04GTPH/file/01HYECK021FCE46HC5SVS91RW1Test |
| حقوق: | No license (in copyright) ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.58F93BEA |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fpls.2024.1384496 |
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