مؤتمر
Neurotoksično dejstvo venoma Vipera ammodytes na modelu izolovane dijafragme pacova ; Neurotoxic effect of Vipera ammodytes venom on the rat isolated diaphragm model
| العنوان: | Neurotoksično dejstvo venoma Vipera ammodytes na modelu izolovane dijafragme pacova ; Neurotoxic effect of Vipera ammodytes venom on the rat isolated diaphragm model |
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| المؤلفون: | Ivanović, Saša, Ćupić, Vitomir, Živković, Irena, Milovanović, Vladimir, Ćupić Miladinović, Dejana, Borozan, Sunčica |
| المصدر: | 13th International Congress of the Serbian Society of Toxicology and 1st TOXSEE Regional Conference, belgrade, 10 - 12 May, 2023 |
| بيانات النشر: | Serbian Society of Toxicology |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Vipera ammodytes, neurotoksičnost, izolovana dijafragma, pacov, neurotoxicity, isolated diaphragm, rats |
| الوصف: | Poskok (Vipera ammodytes) predstavlja najzastupljeniju zmiju otrovnicu u Srbiji i na Balkanu, kako po brojnosti tako i po arealu rasprostranjenosti. U poređenju sa venomima ostalih otrovnica iz familije Viperidae na Balkanu, toksičnost venoma poskoka je najveća. Neurotoksičnost sirovog venoma poskoka smo ispitivali u in vitro uslovima na osnovu kontrakcija preparata dijafragme izazvanih poljnom električnom stimulacijom i aktivnosti enzima: acetilholinesteraze (AChE) i ukupnih ATP-aza u dijafragmi. Formirano je 5 grupa pacova (n=5): kontrola (dijafragma bez venoma), dijafragma sa venomom, dijafragma sa smešom venom/antivenom („Viekvin“, Torlak, Srbija) u odnosima 1:2, 1:10 i 1:20 (m/m). Kontrakcije dijafragme su opale na 50% kontrolnih kontrakcija: pod uticajem venoma za 62,00±2,31 minuta, a sa smešom venom/antivenom 1:2 za 78,50±7,51 minuta, 1:10 za 150,00±17,32 minuta, 1:20 za 307,50±2,89 minuta. Statistička razlika (p0,05) pod dejstvom venoma. Venom inhibira aktivnost ukupnih ATP-aza dijafragme za 51,81% u odnosu na kontrolu. Venom poskoka ne deluje na AChE i nikotinske receptore u neuro-mišićnoj sinapsi dijafragme, već narušava energetski metabolizam mitohondrija. ; Vipera ammodytes is the most common venomous snake in Serbia and the Balkans, both in terms of numbers and area of distribution. Compared to the venoms of other poisonous snakes from the family Viperidae in the Balkans, the toxicity of the V. ammodytes venom is the highest. We investigated the neurotoxicity of crude venom in vitro using contractions of the diaphragm preparation induced by electric field stimulation and enzyme activity: acetylcholinesterase (AChE) and total ATPases in the diaphragm. Five groups of rats were formed (n=5): control (diaphragm without venom), diaphragm with venom, diaphragm with mixture of venom/antivenom ("Viekvin", Torlak, Serbia) in the ratio 1:2, 1:10 and 1:20 (m/m). Diaphragmatic contractions decreased to 50% of control contractions: under the influence of venom for 62.00±2.31 minutes, and with venom/antivenom ... |
| نوع الوثيقة: | conference object |
| اللغة: | Serbian English |
| ردمك: | 978-86-917867-3-1 86-917867-3-6 |
| العلاقة: | https://vet-erinar.vet.bg.ac.rs/handle/123456789/2960Test; http://veterinar.vet.bg.ac.rs/bitstream/id/8716/bitstream_8716.pdfTest; https://hdl.handle.net/21.15107/rcub_veterinar_2960Test |
| الإتاحة: | https://vet-erinar.vet.bg.ac.rs/handle/123456789/2960Test http://veterinar.vet.bg.ac.rs/bitstream/id/8716/bitstream_8716.pdfTest https://hdl.handle.net/21.15107/rcub_veterinar_2960Test |
| حقوق: | openAccess ; https://creativecommons.org/licenses/by/4.0Test/ ; BY |
| رقم الانضمام: | edsbas.58923AE1 |
| قاعدة البيانات: | BASE |
| ردمك: | 9788691786731 8691786736 |
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