دورية أكاديمية
The Effect of Cilostamide on Gap Junction Communication Dynamics, Chromatin Remodeling, and Competence Acquisition in Pig Oocytes Following Parthenogenetic Activation and Nuclear Transfer
| العنوان: | The Effect of Cilostamide on Gap Junction Communication Dynamics, Chromatin Remodeling, and Competence Acquisition in Pig Oocytes Following Parthenogenetic Activation and Nuclear Transfer |
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| المؤلفون: | Dieci C, Lodde V, Franciosi F, Lagutina I, Tessaro I, Modina SC, Albertini DF, Luciano AM, LAZZARI, GIOVANNA, GALLI, CESARE |
| المساهمون: | Dieci C, Lodde V, Franciosi F, Lagutina I, Tessaro I, Modina SC, Albertini DF, Lazzari G, Galli C, Luciano AM |
| سنة النشر: | 2013 |
| المجموعة: | IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
| مصطلحات موضوعية: | Cilostamide, Gap Junction Communication Dynamic, Pig oocyte, Chromatin Remodeling, Parthenogenetic Activation, NUCLEAR TRANSFER |
| الوصف: | In the pig, the efficiency of in vitro embryo production and somatic cell nuclear transfer (SCNT) procedures remains limited. It has been suggested that prematuration treatments (pre-IVM) based on the prolongation of a patent, bidirectional crosstalk between the oocyte and the cumulus cells through gap junction mediate communication (GJC), with the maintenance of a proper level of cAMP, could improve the developmental capability of oocytes. The aim of this study was to assess: 1) dose-dependent effects of cilostamide on nuclear maturation kinetics, 2) the relationship between treatments on GJC functionality and large-scale chromatin configuration changes, and 3) the impact of treatments on developmental competence acquisition after parthenogenetic activation (PA) and SCNT. Accordingly, cumulus-oocyte complexes were collected from 3- to 6-mm antral follicles and cultured for 24 h in defined culture medium with or without 1 μM cilostamide. GJC functionality was assessed by Lucifer yellow microinjection, while chromatin configuration was evaluated by fluorescence microscopy after nuclear staining. Cilostamide administration sustained functional coupling for up to 24 h of culture and delayed meiotic resumption, as only 25.6% of cilostamide-treated oocytes reached the pro-metaphase I stage compared to the control (69.7%; P < 0.05). Moreover, progressive chromatin condensation was delayed before meiotic resumption based upon G2/M biomarker phosphoprotein epitope acquisition using immunolocalization. Importantly, cilostamide treatment under these conditions improved oocyte developmental competence, as reflected in higher blastocyst quality after both parthenogenetic activation and SCNT. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/wos/WOS:000325007200019; volume:89; firstpage:68; lastpage:68; numberofpages:1; journal:BIOLOGY OF REPRODUCTION; http://hdl.handle.net/11585/397599Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84889589378 |
| DOI: | 10.1095/biolreprod.113.110577 |
| الإتاحة: | https://doi.org/10.1095/biolreprod.113.110577Test http://hdl.handle.net/11585/397599Test |
| رقم الانضمام: | edsbas.58145CC |
| قاعدة البيانات: | BASE |
| DOI: | 10.1095/biolreprod.113.110577 |
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