التفاصيل البيبلوغرافية
| العنوان: |
UHPLC-QQQ-MS/MS assay for the quantification of dianthrones as potential toxic markers of Polygonum multiflorum Thunb: applications for the standardization of traditional Chinese medicines (TCMs) with endogenous toxicity |
| المؤلفون: |
Yang, Jian-Bo, Song, Yun-Fei, Liu, Yue, Gao, Hui-Yu, Wang, Qi, Wang, Ying, Cheng, Xian-Long, Zuo, Tian-Tian, Hu, Xiao-Wen, Wei, Feng, Jin, Hong-Tao, Wang, Shu-Ting, Ma, Shuang-Cheng |
| المساهمون: |
National Natural Science Foundation of China, Hubei Technological Innovation Special Fund |
| المصدر: |
Chinese Medicine ; volume 16, issue 1 ; ISSN 1749-8546 |
| بيانات النشر: |
Springer Science and Business Media LLC |
| سنة النشر: |
2021 |
| مصطلحات موضوعية: |
Complementary and alternative medicine, Pharmacology |
| الوصف: |
Background The raw and processed roots of Polygonum multiflorum Thunb (PM) are commonly used in clinical practice to treat diverse diseases; however, reports of hepatotoxicity induced by Polygoni Multiflori Radix (PMR) and Polygoni Multiflori Radix Praeparata (PMRP) have emerged worldwide. Thus, it is necessary for researchers to explore methods to improve quality standards to ensure their quality and treatment effects. Methods In the present study, an ultra-high performance liquid chromatography triple quadrupole mass spectrometry (UHPLC-QQQ-MS/MS) method was optimized and validated for the determination of dianthrones in PMR and PMRP using bianthronyl as the internal standard. Chromatographic separation with a gradient mobile phase [A: acetonitrile and B: water containing 0.1% formic acid (v/v)] at a flow rate of 0.25 mL/min was achieved on an Agilent ZORBAX SB-C 18 column (2.1 mm × 50 mm, 1.8 μm). The triple quadrupole mass spectrometer (TQMS) was operated in negative ionization mode with multiple reaction monitoring for the quantitative analysis of six dianthrones. Moreover, compounds 5 and 6 were further evaluated for their cytotoxicity in HepaRG cells by CCK-8 assay. Results The UHPLC-QQQ-MS/MS method was first developed to simultaneously determine six dianthrones in PMR and PMRP, namely, polygonumnolides C1 – C4 ( 1–4 ), trans -emodin dianthrones ( 5 ), and cis -emodin dianthrones ( 6 ). The contents of 1 – 6 in 90 batches of PMR were in the ranges of 0.027–19.04, 0.022–13.86, 0.073–15.53, 0.034–23.35, 0.38–83.67 and 0.29–67.00 µg/g, respectively. The contents of 1 – 6 in 86 batches of commercial PMRP were in the ranges of 0.020–13.03, 0.051–8.94, 0.022–7.23, 0.030–12.75, 0.098–28.54 and 0.14–27.79 µg/g, respectively. Compounds 1 – 4 were almost completely eliminated after reasonable processing for 24 h and the contents of compounds 5 and 6 significantly decreased. Additionally, compounds 5 and 6 showed inhibitory activity in HepaRG cells with IC 50 values of 10.98 and 15.45 μM, respectively. ... |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1186/s13020-021-00463-w |
| DOI: |
10.1186/s13020-021-00463-w.pdf |
| DOI: |
10.1186/s13020-021-00463-w/fulltext.html |
| الإتاحة: |
https://doi.org/10.1186/s13020-021-00463-wTest |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: |
edsbas.57FE58DC |
| قاعدة البيانات: |
BASE |
