دورية أكاديمية
Per os administered refined olive oil and marine PUFA-rich oils reach the cornea: possible role on oxidative stress through caveolin-1 modulation
| العنوان: | Per os administered refined olive oil and marine PUFA-rich oils reach the cornea: possible role on oxidative stress through caveolin-1 modulation |
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| المؤلفون: | Dutot, Melody, Liang, Hong, Martin, Chantal, Rousseau, Delphine, Grynberg, Alain, Warnet, Jean-Michel, Rat, Patrice |
| المساهمون: | Université Paris Descartes - Paris 5 (UPD5), La Vision, Nutrition Lipidique et Régulation Fonctionnelle du Coeur et des Vaisseaux (NLRFC), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11), Faculté de pharmacie, Université Paris-Sud - Paris 11 (UP11) |
| المصدر: | ISSN: 1743-7075 ; Nutrition and Metabolism ; https://hal.inrae.fr/hal-02668144Test ; Nutrition and Metabolism, 2009, 6, pp.1-9. ⟨10.1186/1743-7075-6-48⟩ ; http://nutritionandmetabolism.biomedcentral.com/articles/10.1186/1743-7075-6-48Test. |
| بيانات النشر: | HAL CCSD BioMed Central |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition |
| الوصف: | International audience ; Olive oil and fish oils are known to possess beneficial properties for human health. We investigated whether different oils and fatty acids alone were able to decrease oxidative stress induced on corneal cells. In our in vivo study, rats were fed with marine oils rich in polyunsaturated fatty acids (PUFA) or refined olive oil during 28 days. At the end of the protocol, corneas were analysed for their fatty acids composition to study the incorporation of fatty acids in cell membranes. In our in vitro study, a human corneal cell line was incubated with marine oils or refined olive oil and subjected to oxidative stress (tBHP 50 muM, 1 hour). Effects on reactive oxygen species generation, mitochondria and caveolin-1 expression were studied using microcytofluorometry, flow cytometry and confocal microscopy. Our results indicate that dietary oils changed the fatty acids composition of corneal cell membranes. According to our results, PUFA-rich oils and refined olive oil (free of antioxidants) blocked reactive oxygen species production. Oleic acid, the major fatty acid of olive oil, also decreased oxidative stress. Moreover, oleic acid modified caveolin-1 expression. Antioxidant properties of oleic acid could be due to disruption of membrane microdomains such as caveolae. Oleic acid, a potential potent modulator of oxidative stress, could be added to PUFA-rich oils to prevent oxidative stress-linked corneal pathology. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/19930652; hal-02668144; https://hal.inrae.fr/hal-02668144Test; https://hal.inrae.fr/hal-02668144/documentTest; https://hal.inrae.fr/hal-02668144/file/2009_Dutot_Nutrition_1Test; PRODINRA: 360820; PUBMED: 19930652; WOS: 000272286400002 |
| DOI: | 10.1186/1743-7075-6-48 |
| الإتاحة: | https://doi.org/10.1186/1743-7075-6-48Test https://hal.inrae.fr/hal-02668144Test https://hal.inrae.fr/hal-02668144/documentTest https://hal.inrae.fr/hal-02668144/file/2009_Dutot_Nutrition_1Test |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.57E49A22 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/1743-7075-6-48 |
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