دورية أكاديمية
CCR4‐NOT differentially controls host versus virus poly(a)‐tail length and regulates HCMV infection
| العنوان: | CCR4‐NOT differentially controls host versus virus poly(a)‐tail length and regulates HCMV infection |
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| المؤلفون: | Burgess, Hannah M, Grande, Rebecca, Riccio, Sofia, Dinesh, Ikshitaa, Winkler, Gerlof Sebastiaan, Depledge, Daniel P, Mohr, Ian |
| بيانات النشر: | EMBO Press |
| سنة النشر: | 2023 |
| المجموعة: | University of Nottingham: Repository@Nottingham |
| مصطلحات موضوعية: | CCR4-NOT, deadenylation, HCMV, RNA decay, virus:host interaction |
| الوصف: | Unlike most RNA and DNA viruses that broadly stimulate mRNA decay and interfere with host gene expression, human cytomegalovirus (HCMV) extensively remodels the host translatome without producing an mRNA decay enzyme. By performing a targeted loss-of-function screen in primary human fibroblasts, we here identify the host CCR4-NOT deadenylase complex members CNOT1 and CNOT3 as unexpected pro-viral host factors that selectively regulate HCMV reproduction. We find that the scaffold subunit CNOT1 is specifically required for late viral gene expression and genome-wide host responses in CCR4-NOT-disrupted cells. By profiling poly(A)-tail lengths of individual HCMV and host mRNAs using nanopore direct RNA sequencing, we reveal poly(A)-tails of viral messages to be markedly longer than those of cellular mRNAs and significantly less sensitive to CCR4-NOT disruption. Our data establish that mRNA deadenylation by host CCR4-NOT is critical for productive HCMV replication and define a new mechanism whereby herpesvirus infection subverts cellular mRNA metabolism to remodel the gene expression landscape of the infected cell. Moreover, we expose an unanticipated host factor with potential to become a therapeutic anti-HCMV target. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 1469-221X |
| العلاقة: | https://nottingham-repository.worktribe.com/output/26262670Test; EMBO Reports; Volume 24; Issue 12; https://nottingham-repository.worktribe.com/file/26262670/1/EMBO%20Reports%20-%202023%20-%20Burgess%20-%20CCR4%E2%80%90NOT%20differentially%20controls%20host%20versus%20virus%20poly%20a%20%E2%80%90tail%20length%20and%20regulates%20HCMVTest |
| DOI: | 10.15252/embr.202256327 |
| الإتاحة: | https://doi.org/10.15252/embr.202256327Test https://nottingham-repository.worktribe.com/file/26262670/1/EMBO%20Reports%20-%202023%20-%20Burgess%20-%20CCR4%E2%80%90NOT%20differentially%20controls%20host%20versus%20virus%20poly%20a%20%E2%80%90tail%20length%20and%20regulates%20HCMVTest https://nottingham-repository.worktribe.com/output/26262670Test |
| حقوق: | openAccess ; https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.579365B |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 1469221X |
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| DOI: | 10.15252/embr.202256327 |