التفاصيل البيبلوغرافية
| العنوان: |
Additional file 3 of Alternative splicing of NF-YA promotes prostate cancer aggressiveness and represents a new molecular marker for clinical stratification of patients |
| المؤلفون: |
Silvia Belluti (104033), Valentina Semeghini (11711427), Giovanna Rigillo (11711430), Mirko Ronzio (8475249), Daniela Benati (190854), Federica Torricelli (7173128), Luca Reggiani Bonetti (11711433), Gianluca Carnevale (117495), Giulia Grisendi (3430946), Alessia Ciarrocchi (37383), Massimo Dominici (3203094), Alessandra Recchia (234598), Diletta Dolfini (90487), Carol Imbriano (11711436) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Medicine, Cell Biology, Genetics, Molecular Biology, Immunology, Developmental Biology, Cancer, Hematology, Infectious Diseases, Space Science, Biological Sciences not elsewhere classified, NF-Y, Prostate cancer, Alternative splicing, Genome editing, Transcriptome profiling |
| الوصف: |
Additional file 3: Suppl. Figure S3. CRISPR/Cas9-mediated knock out of endogenous hNF-YA. (A) Schematic representation of CRISPR/Cas9 strategy to knock down human NF-YA. The picture illustrates the sgRNA targeting endogenous hNF-YA (blue line) and the PAM sequence specific for the human gene (red line). Sequence alignment of the reverse complementary strand of exon 2 of human and mouse NF-YA gene is shown. (B) Indel spectrum determined by TIDE analysis on human NF-YA gene in a representative experiment of bulk CRISPR-treated PC3 cells overexpressing murine NF-YAl (left panel) or NF-YAs (right panel). Editing frequencies are shown (p < 0.05). (C) Indel spectrum determined by TIDE analysis on murine NF-YA in CRISPR-treated and GFP-sorted PC3 cells overexpressing murine NF-YAl (left panel) or NF-YAs (right panel). (D) Indel spectrum determined by TIDE analysis on human NF-YA gene in PC3 NF-YAs clone #11 (left panel) or #23 (right panel). Frequencies of editing are reported (p < 0.05) and show biallelic editing. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/figure/Additional_file_3_of_Alternative_splicing_of_NF-YA_promotes_prostate_cancer_aggressiveness_and_represents_a_new_molecular_marker_for_clinical_stratification_of_patients/17021043Test |
| DOI: |
10.6084/m9.figshare.17021043.v1 |
| الإتاحة: |
https://doi.org/10.6084/m9.figshare.17021043.v1Test |
| حقوق: |
CC BY + CC0 |
| رقم الانضمام: |
edsbas.56AE1C73 |
| قاعدة البيانات: |
BASE |
