دورية أكاديمية
Allergy / IgG4 drives M2a macrophages to a regulatory M2b‐like phenotype: potential implication in immune tolerance
| العنوان: | Allergy / IgG4 drives M2a macrophages to a regulatory M2b‐like phenotype: potential implication in immune tolerance |
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| المؤلفون: | Jensen-Jarolim, Erika, Bianchini, Rodolfo, Roth-Walter, Franziska, Ohradanova-Repic, Anna, Flicker, Sabine, Hufnagl, Karin, Fischer, Michael Bernhard, Stockinger, Hannes |
| بيانات النشر: | Wiley |
| سنة النشر: | 2019 |
| المجموعة: | MedUni Vienna ePub (Medzinische Universität Wien) |
| مصطلحات موضوعية: | allergy, CCL 1, IgG4, immune tolerance, M2b macrophages |
| جغرافية الموضوع: | UMW:14528, UMW:14603 |
| الوصف: | Background Macrophages can be converted in vitro into immunoregulatory M2b macrophages in the presence of immune complexes (IC s), but the role of the specific subclasses IgG1 or IgG4 in this phenotypic and functional change is not known. Objective We aimed to refine the original method by applying precisely defined IC s of the subclasses IgG4 or IgG1 constructed by two independent methods. Methods Monocyte‐derived macrophages (MDM s) were treated with M‐CSF , followed by IL ‐4/IL ‐13 to induce the M2a allergic phenotype. To mimic unspecific or allergen‐specific IC s, plates were coated with myeloma IgG1 or IgG4, or with grass pollen allergen Phl p 5 followed by recombinant human Phl p 5‐specific IgG1 or IgG4. M2a polarized macrophages were then added, cultured, and examined for cellular markers and cytokines by flow cytometry, ELISA , and rtPCR . Alternatively, immune complexes with IgG1 or IgG4 were formed using protein L. Results IgG4 IC s down regulated CD 163 and CD 206 on M2a cells, and significantly increased IL ‐10, IL ‐6, TNF α, and CCL 1 secretion, indicating a shift to an M2b‐like phenotype. Treatment with IgG4 IC s resulted in expression of FcγRII and down modulation of FcγRII compared with IgG1 treated cells (P = 0.0335) or untreated cells (P < 0.00001). Conclusion Immune complexes with subclasses IgG1 and IgG4 can in vitro be generated by plate absorption, and in fluid form by protein L. Cross‐linking of FcγRII b by the IgG4 subclass redirects pro‐allergic M2a macrophages to an M2b‐like immunosuppressive phenotype. This suggests an interplay of macrophages with IgG4 in immune tolerance, likely relevant in allergen immunotherapy. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text/html |
| اللغة: | English |
| تدمد: | 1398-9995 |
| العلاقة: | vignette : https://repositorium.meduniwien.ac.at/titlepage/urn/urn:nbn:at:at-ubmuw:3-23194/128Test; urn:nbn:at:at-ubmuw:3-23194; https://resolver.obvsg.at/urn:nbn:at:at-ubmuw:3-23194Test; local:99145452300703331; system:AC15657634 |
| DOI: | 10.1111/all.13635 |
| الإتاحة: | https://doi.org/10.1111/all.13635Test https://resolver.obvsg.at/urn:nbn:at:at-ubmuw:3-23194Test |
| حقوق: | cc-by_4 |
| رقم الانضمام: | edsbas.5656077 |
| قاعدة البيانات: | BASE |
| تدمد: | 13989995 |
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| DOI: | 10.1111/all.13635 |