دورية أكاديمية
External quality assessment of noninvasive fetal RHD genotyping
| العنوان: | External quality assessment of noninvasive fetal RHD genotyping |
|---|---|
| المؤلفون: | Clausen, Frederik Banch, Hellberg, Åsa |
| المصدر: | Vox Sanguinis; 115(5), pp 466-471 (2020) ; ISSN: 0042-9007 |
| بيانات النشر: | Wiley-Blackwell |
| سنة النشر: | 2020 |
| المجموعة: | Lund University Publications (LUP) |
| مصطلحات موضوعية: | Clinical Laboratory Medicine, cell-free fetal DNA, external quality assessment, fetal RHD genotyping, Rh prophylaxis |
| الوصف: | Background and objectives: Fetal RHD genotyping of cell-free maternal plasma DNA from RhD negative pregnant women can be used to guide targeted antenatal and postnatal anti-D prophylaxis for the prevention of RhD immunization. To assure the quality of clinical testing, we conducted an external quality assessment workshop with the participation of 31 laboratories. Materials and methods: Aliquots of pooled maternal plasma from gestational week 25 were sent to each laboratory. One sample was fetal RHD positive, and a second sample was fetal RHD negative. A reporting scheme was supplied for data collection, including questions regarding the methodological setup, results and clinical recommendations. The samples were tested blindly. Results: Different methodological approaches were used; 29 laboratories used qPCR and two laboratories used ddPCR, employing a total of eight different combinations of RHD exon targets. Fetal RHD genotyping was performed with no false-negative and no false-positive results. One inconclusive result was reported for the RHD positive sample. All clinical conclusions were satisfactory. Conclusion: This external quality assessment workshop demonstrates that despite the different approaches taken to perform the clinical assays, fetal RHD genotyping is a reliable laboratory assay to guide targeted use of Rh prophylaxis in a clinical setting. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://lup.lub.lu.se/record/49d56848-a6f2-4f30-b729-5d8b2d137775Test; http://dx.doi.org/10.1111/vox.12908Test; scopus:85081750187; pmid:32166750 |
| DOI: | 10.1111/vox.12908 |
| الإتاحة: | https://doi.org/10.1111/vox.12908Test https://lup.lub.lu.se/record/49d56848-a6f2-4f30-b729-5d8b2d137775Test |
| رقم الانضمام: | edsbas.56280828 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/vox.12908 |
|---|