التفاصيل البيبلوغرافية
| العنوان: |
Thymic expression of peripheral tissue antigens in humans: a remarkable variability among individuals |
| المؤلفون: |
Hiroshi Takase, Cheng-rong Yu, Rashid M. Mahdi, Daniel C. Douek, Gregory B, Frank M. Midgley, Rajpreet Dogra, Gloria Allende, Eliot Rosenkranz, Charles E. Egwuagu, Igal Gery |
| المساهمون: |
The Pennsylvania State University CiteSeerX Archives |
| المصدر: |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2366090/pdf/nihms-44922.pdfTest. |
| سنة النشر: |
2005 |
| المجموعة: |
CiteSeerX |
| مصطلحات موضوعية: |
retinal antigen, susceptibility |
| الوصف: |
The majority of maturing T lymphocytes that recognize self-antigens is eliminated in the thymus upon exposure to their target antigens. This physiological process of negative selection requires that tissue-specific antigens be expressed by thymic cells, a phenomenon that has been well studied in experimental animals. Here, we have examined the expression in human thymi of four retinal antigens, that are capable of inducing autoimmune ocular disease retinal S-antigen (S-Ag), recoverin, RPE65 and inter-photoreceptor retinoid-binding protein (IRBP)], as well as four melanocyte-specific antigens, two of which are used as targets for melanoma immunotherapy [gp100, melanoma antigen recognized by T cells 1, tyrosinase-related protein (TRP)-1 and TRP-2]. Using reverse transcription (RT)–PCR, we found that all thymic samples from the 18 donors expressed mRNA transcripts of most or all the eight tested tissue antigens. Yet, the expression of the transcripts varied remarkably among the individual thymic samples. In addition, S-Ag, RPE65 and IRBP were detected by immunostaining in rare cells in sections of human thymi by antibodies against these proteins. Quantitative real-time RT–PCR analysis revealed that the retinal antigen transcripts in the human thymus are present at trace levels, that are lower by approximately five orders of magnitude than |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.661.4845Test; http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2366090/pdf/nihms-44922.pdfTest |
| الإتاحة: |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2366090/pdf/nihms-44922.pdfTest |
| حقوق: |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: |
edsbas.54D0F09A |
| قاعدة البيانات: |
BASE |
