دورية أكاديمية
Anti-herpetic activity of a sulfated xylomannan from Scinaia hatei
| العنوان: | Anti-herpetic activity of a sulfated xylomannan from Scinaia hatei |
|---|---|
| المؤلفون: | Mandal, P., Pujol, C.A., Carlucci, M.J., Chattopadhyay, K., Damonte, E.B., Ray, B. |
| المجموعة: | Biblioteca Digital FCEN-UBA (Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires) |
| مصطلحات موضوعية: | Antiviral activity, Herpes simplex virus, Liagoraceae, Scinaia hatei, Xylomannan sulfate, anticoagulant agent, antivirus agent, glycoside, mannan, sulfate, animal, article, Cercopithecus, chemistry, drug effect, gel chromatography, Herpes simplex virus 1, Herpes simplex virus 2, human, isolation and purification, nuclear magnetic resonance spectroscopy, red alga, Vero cell, Algae, Red, Animals, Anticoagulants, Antiviral Agents, Cercopithecus aethiops, Chromatography |
| الوصف: | Many viruses display affinity for cell surface heparan sulfate proteoglycans with biological relevance in virus entry. This raises the possibility of the application of sulfated polysaccharides in antiviral therapy. In this study we have analyzed polysaccharide fractions isolated from Scinaia hatei. The crude water extract (ShWE) as well as one fraction (F1) obtained by size exclusion chromatography had potent anti-HSV activity. Their inhibitory concentration 50% (IC50) values ranging from 0.5 to 4.6 μg/ml were much lower than the cytotoxic concentration 50% (CC50) values (≥1000 μg/ml). These fractions had very low anticoagulant activity. Furthermore, they had a weak inactivating effect on virions in a virucidal assay at concentrations in the range of 60-100 μg/ml. Chemical, chromatographic and spectroscopic methods showed that the major polysaccharide, which had 0.4 sulfate group per monomer unit and an apparent molecular mass of 160 kDa, contained a backbone of α-(1 → 3)-linked d-mannopyranosyl residues substituted at C-6, C-4 and C-2 with single stub of β-d-xylopyranosyl residues. Sulfate groups, when present, are located at C-4 of α-(1 → 3)-linked d-mannopyranosyl units, and appeared to be very important for the anti-herpetic activity of this polymer. © 2008 Elsevier Ltd. All rights reserved. ; Fil:Pujol, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. ; Fil:Carlucci, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. ; Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | unknown |
| العلاقة: | http://hdl.handle.net/20.500.12110/paper_00319422_v69_n11_p2193_MandalTest |
| الإتاحة: | https://doi.org/20.500.12110/paper_00319422_v69_n11_p2193_MandalTest https://hdl.handle.net/20.500.12110/paper_00319422_v69_n11_p2193_MandalTest |
| حقوق: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/2.5/arTest |
| رقم الانضمام: | edsbas.542E5069 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |