دورية أكاديمية
Androgen receptor-mediated apoptosis in bovine testicular induced pluripotent stem cells in response to phthalate esters
| العنوان: | Androgen receptor-mediated apoptosis in bovine testicular induced pluripotent stem cells in response to phthalate esters |
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| المؤلفون: | Wang, S-W, Wang, S S-W, Wu, D-C, Lin, Y-C, Ku, C-C, Wu, C-C, Chai, C-Y, Lee, J-N, Tsai, E-M, Lin, C-LS, Yang, R-C, Ko, Y-C, Yu, H-S, Huo, C, Chuu, C-P, Murayama, Y, Nakamura, Y, Hashimoto, S, Matsushima, K, Jin, C, Eckner, R, Lin, C-S, Saito, S, Yokoyama, K K |
| المصدر: | Cell Death & Disease ; volume 4, issue 11, page e907-e907 ; ISSN 2041-4889 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Cancer Research, Cell Biology, Cellular and Molecular Neuroscience, Immunology |
| الوصف: | The androgen receptor (AR) has a critical role in promoting androgen-dependent and -independent apoptosis in testicular cells. However, the molecular mechanisms that underlie the ligand-independent apoptosis, including the activity of AR in testicular stem cells, are not completely understood. In the present study, we generated induced pluripotent stem cells (iPSCs) from bovine testicular cells by electroporation of octamer-binding transcription factor 4 ( OCT4 ). The cells were supplemented with leukemia inhibitory factor and bone morphogenetic protein 4, which maintained and stabilized the expression of stemness genes and pluripotency. The iPSCs were used to assess the apoptosis activity following exposure to phthalate esters, including di (2-ethyhexyl) phthalates, di ( n -butyl) phthalate, and butyl benzyl phthalate. Phthalate esters significantly reduced the expression of AR in iPSCs and induced a higher ratio of BAX/BCL-2, thereby favoring apoptosis. Phthalate esters also increased the expression of cyclin-dependent kinase inhibitor 1 (p21 Cip1 ) in a p53-dependent manner and enhanced the transcriptional activity of p53. The forced expression of AR and knockdown of p21 Cip1 led to the rescue of the phthalate-mediated apoptosis. Overall, this study suggests that testicular iPSCs are a useful system for screening the toxicity of environmental disruptors and examining their effect on the maintenance of stemness and pluripotency, as well as for identifying the iPSC signaling pathway(s) that are deregulated by these chemicals. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/cddis.2013.420 |
| الإتاحة: | https://doi.org/10.1038/cddis.2013.420Test http://www.nature.com/articles/cddis2013420.pdfTest http://www.nature.com/articles/cddis2013420Test https://www.nature.com/articles/cddis2013420.pdfTest |
| حقوق: | http://creativecommons.org/licenses/by/3.0Test |
| رقم الانضمام: | edsbas.5325773 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/cddis.2013.420 |
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