دورية أكاديمية
The THP1-SAC3-SUS1-CDC31 complex works in transcription elongation-mRNA export preventing RNA-mediated genome instability
| العنوان: | The THP1-SAC3-SUS1-CDC31 complex works in transcription elongation-mRNA export preventing RNA-mediated genome instability |
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| المؤلفون: | González Aguilera, Cristina, Tous Rivera, Cristina, Gómez González, Belén, Huertas Sánchez, Pablo, Luna Varo, Rosa María, Aguilera López, Andrés |
| المساهمون: | Universidad de Sevilla. Departamento de Genética |
| بيانات النشر: | American Society for Cell Biology |
| سنة النشر: | 2015 |
| المجموعة: | idUS - Deposito de Investigación Universidad de Sevilla |
| مصطلحات موضوعية: | Cell Cycle Proteins/metabolism, Genomic Instability, Nuclear Proteins/metabolism, RNA, Messenger/metabolism, RNA-Binding Proteins/metabolism, Ribonucleoproteins/metabolism, Saccharomyces cerevisiae/physiology, Saccharomyces cerevisiae Proteins/metabolism, Transcription, Genetic, Calcium-Binding Proteins, metabolism |
| الوصف: | The eukaryotic THO/TREX complex, involved in mRNP biogenesis, plays a key role in the maintenance of genome integrity in yeast. mRNA export factors such as Thp1-Sac3 also affect genome integrity, but their mutations have other phenotypes different from those of THO/TREX. Sus1 is a novel component of SAGA transcription factor that also associates with Thp1-Sac3, but little is known about its effect on genome instability and transcription. Here we show that Thp1, Sac3, and Sus1 form a functional unit with a role in mRNP biogenesis and maintenance of genome integrity that is independent of SAGA. Importantly, the effects of ribozyme-containing transcription units, RNase H, and the action of human activation-induced cytidine deaminase on transcription and genome instability are consistent with the possibility that R-loops are formed in Thp1-Sac3-Sus1-Cdc31 as in THO mutants. Our data reveal that Thp1-Sac3-Sus1-Cdc31, together with THO/TREX, define a specific pathway connecting transcription elongation with export via an RNA-dependent dynamic process that provides a feedback mechanism for the control of transcription and the preservation of genetic integrity of transcribed DNA regions. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | Molecular Biology of the Cell, 19(10), 4310-4318; http://dx.doi.org/10.1091/mbc.E08-04-0355Test; https://idus.us.es/handle/11441/29169Test |
| الإتاحة: | https://doi.org/10.1091/mbc.E08-04-0355Test https://idus.us.es/handle/11441/29169Test |
| حقوق: | Attribution-NonCommercial-NoDerivatives 4.0 Internacional ; http://creativecommons.org/licenses/by-nc-nd/4.0Test/ ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.53245DEC |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |