دورية أكاديمية
A monocyte/dendritic cell molecular signature of SARS-CoV-2-related multisystem inflammatory syndrome in children with severe myocarditis
| العنوان: | A monocyte/dendritic cell molecular signature of SARS-CoV-2-related multisystem inflammatory syndrome in children with severe myocarditis |
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| المؤلفون: | de Cevins, Camille, Luka, Marine, Smith, Nikaïa, Meynier, Sonia, Magérus, Aude, Carbone, Francesco, García-Paredes, Víctor, Barnabei, Laura, Batignes, Maxime, Boullé, Alexandre, Stolzenberg, Marie-Claude, Pérot, Brieuc, P., Charbit, Bruno, Fali, Tinhinane, Pirabakaran, Vithura, Sorin, Boris, Riller, Quentin, Abdessalem, Ghaith, Beretta, Maxime, Grzelak, Ludivine, Goncalves, Pedro, Di Santo, James, P., Mouquet, Hugo, Schwartz, Olivier, Zarhrate, Mohammed, Parisot, Mélanie, Bole-Feysot, Christine, Masson, Cécile, Cagnard, Nicolas, Corneau, Aurélien, Brunaud, Camille, Zhang, Shen-Ying, Casanova, Jean-Laurent, Bader-Meunier, Brigitte, Haroche, Julien, Melki, Isabelle, Lorrot, Mathie, Oualha, Mehdi, Moulin, Florence, Bonnet, Damien, Belhadjer, Zahra, Leruez, Marianne, Allali, Slimane, Gras-Leguen, Christèle, de Pontual, Loïc, Study Group, Pediatric-Biocovid, Fischer, Alain, Duffy, Darragh, Rieux-Laucat, Fredéric, Toubiana, Julie, Ménager, Mickaël |
| المساهمون: | Inflammatory Responses and Transcriptomic Networks in diseases (Equipe Inserm U1163), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Sanofi Aventis R&D Chilly-Mazarin, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Immunologie Translationnelle - Translational Immunology lab, Institut Pasteur Paris (IP), Immunogenetics of pediatric autoimmune diseases (Equipe Inserm U1163), Cytometrie et Biomarqueurs – Cytometry and Biomarkers (UTechS CB), Immunologie humorale - Humoral Immunology, Institut Pasteur Paris (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Virus et Immunité - Virus and immunity (CNRS-UMR3569), Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS), Immunité Innée - Innate Immunity, Structure Fédérative de Recherche Necker (UAR 3633 / US24), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Cytométrie Pitié-Salpêtrière (PASS-CYPS), Unité Mixte de Service Production et Analyse de données en Sciences de la vie et en Santé (PASS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Human genetics of infectious diseases : Mendelian predisposition (Equipe Inserm U1163), Rockefeller University New York, Hôpital Necker - Enfants Malades AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), CHU Pitié-Salpêtrière AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), AP-HP Hôpital universitaire Robert-Debré Paris, Université Paris Cité (UPCité), CHU Trousseau APHP, Centre d’Investigation Clinique de Nantes (CIC Nantes), Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Universitaire de Nantes = Nantes University Hospital (CHU Nantes), Hôpital Jean Verdier AP-HP, Université Paris 13 (UP13), Collège de France (CdF (institution)), Biodiversité et Epidémiologie des Bactéries pathogènes - Biodiversity and Epidemiology of Bacterial Pathogens, ANR-18-IDEX-0001,Université de Paris,Université de Paris(2018), ANR-17-EURE-0013,GENE,Génétique et Epigénétique Nouvelle Ecole(2017), ANR-18-RHUS-0010,ATRACTION,Autoimmunity/inflammation Through Rnaseq Analysis at the single Cell level for Therapeutic Innovation(2018) |
| المصدر: | ISSN: 2666-6340 ; Med ; https://hal.sorbonne-universite.fr/hal-03590894Test ; Med, 2021, 2 (9), pp.1072-1092.e7. ⟨10.1016/j.medj.2021.08.002⟩. |
| بيانات النشر: | HAL CCSD Cell Press |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | COVID-19, SARS-CoV-2, scRNA-seq, MIS-C, PIMS-TS, Kawasaki Disease, myocarditis, TNF-α and NF-κB signaling, lack of responses to type I and type II IFN secretion, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; BackgroundSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in children is generally milder than in adults, but a proportion of cases result in hyperinflammatory conditions often including myocarditis.MethodsTo better understand these cases, we applied a multiparametric approach to the study of blood cells of 56 children hospitalized with suspicion of SARS-CoV-2 infection. Plasma cytokine and chemokine levels and blood cellular composition were measured, alongside gene expression at the bulk and single-cell levels.FindingsThe most severe forms of multisystem inflammatory syndrome in children (MIS-C) related to SARS-CoV-2 that resulted in myocarditis were characterized by elevated levels of pro-angiogenesis cytokines and several chemokines. Single-cell transcriptomics analyses identified a unique monocyte/dendritic cell gene signature that correlated with the occurrence of severe myocarditis characterized by sustained nuclear factor κB (NF-κB) activity and tumor necrosis factor alpha (TNF-α) signaling and associated with decreased gene expression of NF-κB inhibitors. We also found a weak response to type I and type II interferons, hyperinflammation, and response to oxidative stress related to increased HIF-1α and Vascular endothelial growth factor (VEGF) signaling.ConclusionsThese results provide potential for a better understanding of disease pathophysiology. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/34414385; hal-03590894; https://hal.sorbonne-universite.fr/hal-03590894Test; https://hal.sorbonne-universite.fr/hal-03590894/documentTest; https://hal.sorbonne-universite.fr/hal-03590894/file/S2666634021002877.pdfTest; PII: S2666-6340(21)00287-7; PUBMED: 34414385; PUBMEDCENTRAL: PMC8363470 |
| DOI: | 10.1016/j.medj.2021.08.002 |
| الإتاحة: | https://doi.org/10.1016/j.medj.2021.08.002Test https://hal.sorbonne-universite.fr/hal-03590894Test https://hal.sorbonne-universite.fr/hal-03590894/documentTest https://hal.sorbonne-universite.fr/hal-03590894/file/S2666634021002877.pdfTest |
| حقوق: | http://creativecommons.org/licenses/by-ncTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.52DA7F76 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.medj.2021.08.002 |
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