التفاصيل البيبلوغرافية
| العنوان: |
Carbon dots as a versatile tool to monitor insulin aggregation |
| المؤلفون: |
Zingale, Gabriele Antonio, Distefano, Alessia, Pandino, Irene, Tuccitto, Nunzio, Oliveri, Valentina, Gaeta, Massimiliano, D’Urso, Alessandro, Arcoria, Alfio, Grasso, Giuseppe |
| المساهمون: |
Lazio Innova, Università di Catania, Università degli Studi di Catania |
| المصدر: |
Analytical and Bioanalytical Chemistry ; volume 415, issue 10, page 1829-1840 ; ISSN 1618-2642 1618-2650 |
| بيانات النشر: |
Springer Science and Business Media LLC |
| سنة النشر: |
2023 |
| مصطلحات موضوعية: |
Biochemistry, Analytical Chemistry |
| الوصف: |
The possibility to monitor peptide and protein aggregation is of paramount importance in the so-called conformational diseases, as the understanding of many physiological pathways, as well as pathological processes involved in the development of such diseases, depends very much on the actual possibility to monitor biomolecule oligomeric distribution and aggregation. In this work, we report a novel experimental method to monitor protein aggregation, based on the change of the fluorescent properties of carbon dots upon protein binding. The results obtained in the case of insulin with this newly proposed experimental approach are compared with those obtained with other common experimental techniques normally used for the same purpose (circular dichroism, DLS, PICUP and ThT fluorescence). The greatest advantage of the hereby presented methodology over all the other experimental methods considered is the possibility to monitor the initial stages of insulin aggregation under the different experimental conditions sampled and the absence of possible disturbances and/or molecular probes during the aggregation process. Graphical abstract |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1007/s00216-023-04585-y |
| DOI: |
10.1007/s00216-023-04585-y.pdf |
| DOI: |
10.1007/s00216-023-04585-y/fulltext.html |
| الإتاحة: |
https://doi.org/10.1007/s00216-023-04585-yTest |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: |
edsbas.51C25FAC |
| قاعدة البيانات: |
BASE |
