دورية أكاديمية
MiRNA-181a regulates Toll-like receptor agonist-induced inflammatory response in human fibroblasts
| العنوان: | MiRNA-181a regulates Toll-like receptor agonist-induced inflammatory response in human fibroblasts |
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| المؤلفون: | Galicia, J C, Naqvi, A R, Ko, C-C, Nares, S, Khan, A A |
| المصدر: | Genes and Immunity, 15(5) |
| سنة النشر: | 2014 |
| المجموعة: | Carolina Digital Repository (UNC - University of North Carolina) |
| مصطلحات موضوعية: | Humans, Cells, Cultured, Lipopolysaccharides, MicroRNAs, MIrn181 microRNA, human, Dental Pulp, Binding Sites, 3' Untranslated Regions, Toll-Like Receptors, Fibroblasts, Interleukin-8 |
| الوصف: | MicroRNAs (miRNA) regulate the synthesis of cytokines in response to Toll-like receptor (TLR) activation. Our recent microarray study comparing normal and inflamed human dental pulps showed that miRNA-181 (miR-181) family is differentially expressed in the presence of inflammation. Prior studies have reported that the dental pulp, which is composed primarily of TLR4/2+ fibroblasts, expresses elevated levels of cytokines including Interleukin-8 (IL-8) when inflamed. In this study, we employed an in-vitro model to determine the role of the miRNA 181 family in the TLR agonist-induced response in human fibroblasts. TLR4/2+ primary human dental pulp fibroblasts were stimulated with lipopolysaccharide from Porphyromonas gingivalis (Pg LPS), a known oral pathogen, and IL-8 and miR-181 expression measured. An inversely proportional relationship between IL-8 and miR-181a was observed. In-silico analysis identified a miR-181a binding site on the 3′UTR of IL-8 which was confirmed by dual-luciferase assays. MiR-181a directly binds to the 3′UTR of IL-8, an important inflammatory component of the immune response, and modulates its levels. This is the very first report demonstrating miR-181a regulation of IL-8. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://doi.org/10.17615/vyzx-wz26Test; https://cdr.lib.unc.edu/downloads/n870zz48q?file=thumbnailTest; https://cdr.lib.unc.edu/downloads/n870zz48qTest |
| DOI: | 10.17615/vyzx-wz26 |
| الإتاحة: | https://doi.org/10.17615/vyzx-wz26Test https://cdr.lib.unc.edu/downloads/n870zz48q?file=thumbnailTest https://cdr.lib.unc.edu/downloads/n870zz48qTest |
| حقوق: | http://rightsstatements.org/vocab/InC/1.0Test/ |
| رقم الانضمام: | edsbas.51B11E8 |
| قاعدة البيانات: | BASE |
| DOI: | 10.17615/vyzx-wz26 |
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