دورية أكاديمية
GM-CSF induces noninflammatory proliferation of microglia and disturbs electrical neuronal network rhythms in situ
| العنوان: | GM-CSF induces noninflammatory proliferation of microglia and disturbs electrical neuronal network rhythms in situ |
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| المؤلفون: | Dikmen, Onur, Hemmerich, Marc, Lewen, Andrea, hollnagel, jan-oliver, Chausse, Bruno, Kann, Oliver |
| المصدر: | http://lobid.org/resources/99370676031606441Test#!, 17(1):235. |
| سنة النشر: | 2020 |
| المجموعة: | Publisso (ZB MED-Publikationsportal Lebenswissenschaften) |
| مصطلحات موضوعية: | Slice culture, Neuroinflammation, Microglia/drug effects [MeSH], Nitric Oxide/metabolism [MeSH], Microglia/metabolism [MeSH], Male [MeSH], Neuronal activity, Innate immunity, Interneurons/metabolism [MeSH], Neurotransmission, Electrophysiology, Tumor Necrosis Factor-alpha/metabolism [MeSH], Hippocampus/metabolism [MeSH], Interleukin-6/metabolism [MeSH], Hippocampus, Microglia, GM-CSF, Rats [MeSH], Animals [MeSH], Rats, Wistar [MeSH], Cytokines, Interneurons/drug effects [MeSH], Research, Hippocampus/drug effects [MeSH], Cell Proliferation/drug effects [MeSH], Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology [MeSH] |
| الوصف: | Background!#!The granulocyte-macrophage colony-stimulating factor (GM-CSF) (or CSF-2) is involved in myeloid cell growth and differentiation, and, possibly, a major mediator of inflammation in body tissues. The role of GM-CSF in the activation of microglia (CNS resident macrophages) and the consequent impacts on neuronal survival, excitability, and synaptic transmission are widely unknown, however. Here, we focused on electrical neuronal network rhythms in the gamma frequency band (30-70 Hz). Gamma oscillations are fundamental to higher brain functions, such as perception, attention, and memory, and they are exquisitely sensitive to metabolic and oxidative stress.!##!Methods!#!We explored the effects of chronic GM-CSF exposure (72 h) on microglia in male rat organotypic hippocampal slice cultures (in situ), i.e., postnatal cortex tissue lacking leukocyte invasion (adaptive immunity). We applied extracellular electrophysiological recordings of local field potential, immunohistochemistry, design-based stereology, biochemical analysis, and pharmacological ablation of microglia.!##!Results!#!GM-CSF triggered substantial proliferation of microglia (microgliosis). By contrast, the release of proinflammatory cytokines (IL-6, TNF-α) and nitric oxide, the hippocampal cytoarchitecture as well as the morphology of parvalbumin-positive inhibitory interneurons were unaffected. Notably, GM-CSF induced concentration-dependent, long-lasting disturbances of gamma oscillations, such as slowing (beta frequency band) and neural burst firing (hyperexcitability), which were not mimicked by the T lymphocyte cytokine IL-17. These disturbances were attenuated by depletion of the microglial cell population with liposome-encapsulated clodronate. In contrast to priming with the cytokine IFN-γ (type II interferon), GM-CSF did not cause inflammatory neurodegeneration when paired with the TLR4 ligand LPS.!##!Conclusions!#!GM-CSF has a unique role in the activation of microglia, including the potential to induce neuronal network dysfunction. ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://repository.publisso.de/resource/frl:6464853Test; https://doi.org/10.1186/s12974-020-01903-4Test; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7418331Test/ |
| DOI: | 10.1186/s12974-020-01903-4 |
| الإتاحة: | https://doi.org/10.1186/s12974-020-01903-4Test https://repository.publisso.de/resource/frl:6464853Test https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7418331Test/ |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.518E7351 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12974-020-01903-4 |
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