التفاصيل البيبلوغرافية
العنوان: |
Exosomal long noncoding RNA MLETA1 promotes tumor progression and metastasis by regulating the miR-186-5p/EGFR and miR-497-5p/IGF1R axes in non-small cell lung cancer |
المؤلفون: |
Hsu, Xiu-Rui, Wu, Jia-En, Wu, Yi-Ying, Hsiao, Sheng-Yen, Liang, Jui-Lin, Wu, Ya-Ju, Tung, Chia-Hao, Huang, Meng-Fan, Lin, Ming-Shiu, Yang, Pan-Chyr, Chen, Yuh-Ling, Hong, Tse-Ming |
المساهمون: |
Ministry of Science and Technology, Taiwan |
المصدر: |
Journal of Experimental & Clinical Cancer Research ; volume 42, issue 1 ; ISSN 1756-9966 |
بيانات النشر: |
Springer Science and Business Media LLC |
سنة النشر: |
2023 |
مصطلحات موضوعية: |
Cancer Research, Oncology |
الوصف: |
Background Lung cancer is the most common and deadliest cancer worldwide, and approximately 90% of all lung cancer deaths are caused by tumor metastasis. Tumor-derived exosomes could potentially promote tumor metastasis through the delivery of metastasis-related molecules. However, the function and underlying mechanism of exosomal long noncoding RNA (lncRNA) in lung cancer metastasis remain largely unclear. Methods Cell exosomes were purified from conditioned media by differential ultracentrifugation and observed using transmission electron microscopy, and the size distributions were determined by nanoparticle tracking analysis. Exosomal lncRNA sequencing (lncRNA-seq) was used to identify long noncoding RNAs. Cell migration and invasion were determined by wound-healing assays, two-chamber transwell invasion assays and cell mobility tracking. Mice orthotopically and subcutaneously xenografted with human cancer cells were used to evaluate tumor metastasis in vivo. Western blot, qRT‒PCR, RNA-seq, and dual-luciferase reporter assays were performed to investigate the potential mechanism. The level of exosomal lncRNA in plasma was examined by qRT‒PCR. MS2-tagged RNA affinity purification (MS2-TRAP) assays were performed to verify lncRNA-bound miRNAs. Results Exosomes derived from highly metastatic lung cancer cells promoted the migration and invasion of lung cancer cells with low metastatic potential. Using lncRNA-seq, we found that a novel lncRNA, lnc-MLETA1, was upregulated in highly metastatic cells and their secreted exosomes. Overexpression of lnc-MLETA1 augmented cell migration and invasion of lung cancer. Conversely, knockdown of lnc-MLETA1 attenuated the motility and metastasis of lung cancer cells. Interestingly, exosome-transmitted lnc-MLETA1 promoted cell motility and metastasis of lung cancer. Reciprocally, targeting lnc-MLETA1 with an LNA suppressed exosome-induced lung cancer cell motility. Mechanistically, lnc-MLETA1 regulated the expression of EGFR and IGF1R by sponging miR-186-5p and ... |
نوع الوثيقة: |
article in journal/newspaper |
اللغة: |
English |
DOI: |
10.1186/s13046-023-02859-y |
DOI: |
10.1186/s13046-023-02859-y.pdf |
DOI: |
10.1186/s13046-023-02859-y/fulltext.html |
الإتاحة: |
https://doi.org/10.1186/s13046-023-02859-yTest |
حقوق: |
https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
رقم الانضمام: |
edsbas.518DAF04 |
قاعدة البيانات: |
BASE |