التفاصيل البيبلوغرافية
| العنوان: |
Ppg1-Far complex mediated gluconeogenic flux regulation enables cells to adapt to glucose depletion. |
| المؤلفون: |
Shreyas Niphadkar, Lavanya Karinje, Sunil Laxman |
| سنة النشر: |
2024 |
| المجموعة: |
Smithsonian Institution: Figshare |
| مصطلحات موضوعية: |
Biochemistry, Microbiology, Cell Biology, Genetics, Cancer, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, saccharomyces cerevisiae <, mediated via regulation, maintain gluconeogenic outputs, localized cytosolic pools, changing nutrient conditions, balance gluconeogenic outputs, appropriately reallocate resources, protein scaffold present, cells reorganize outputs, assembled far complex, ppg1 catalytic activity, like phosphatase ppg1, reversible protein phosphorylation, div >< p, far complex —, far complex assembly, enable metabolic adaptation, far complex, protein dephosphorylation, subunit complex, phosphorylation status, glcnac —, enables adaptation |
| الوصف: |
A) A schematic describing the experimental details of growth competition experiment. WT cells expressing mCherry and ppg1Δ cells expressing mNeonGreen were mixed in equal proportion. These cells were grown in YPD medium and after growth for the indicated time were shifted to fresh YPD medium. The number of WT and ppg1Δ cells were calculated by fluorescence microscopy. B) Growth competition between WT and ppg1Δ cells in changing glucose conditions. The total culturing time for the competition experiment was 96 hours. Data represented as a mean ± SD (n = 3). Also, see S7A Fig for a similar growth competition between WT and Far9ΔTA10ΔTA cells. C) Growth competition between WT and ppg1Δ cells in glucose-replete conditions. The total culturing time for the competition experiment was 24 hours. Data represented as a mean ± SD (n = 3). D) Comparative growth of WT and ppg1Δ cells in YPD medium. The cultures of WT and ppg1Δ were started at OD 600 of 0.2 in YPD medium and the growth was monitored. Also, see S7B Fig for a similar growth analysis of WT and far11Δ cells under the same conditions. E) Proposed model. Ppg1 phosphatase controls the assembly of the Far complex irrespective of glucose availability. Glucose availability regulates amounts of Far complex. Ppg1-Far complex controls carbon flux and gluconeogenic outputs specifically in the low glucose. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/figure/Ppg1-Far_complex_mediated_gluconeogenic_flux_regulation_enables_cells_to_adapt_to_glucose_depletion_/25365279Test |
| DOI: |
10.1371/journal.pgen.1011202.g007 |
| الإتاحة: |
https://doi.org/10.1371/journal.pgen.1011202.g007Test |
| حقوق: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.515E334E |
| قاعدة البيانات: |
BASE |
