دورية أكاديمية

Rice‐produced classical swine fever virus glycoprotein E2 with herringbone‐dimer design to enhance immune responses

التفاصيل البيبلوغرافية
العنوان: Rice‐produced classical swine fever virus glycoprotein E2 with herringbone‐dimer design to enhance immune responses
المؤلفون: Xu, Qianru, Ma, Fanshu, Yang, Daichang, Li, Qingmei, Yan, Liming, Ou, Jiquan, Zhang, Longxian, Liu, Yunchao, Zhan, Quan, Li, Rui, Wei, Qiang, Hu, Hui, Wang, Yanan, Li, Xueyang, Zhang, Shenli, Yang, Jifei, Chai, Shujun, Du, Yongkun, Wang, Li, Zhang, Erqin, Zhang, Gaiping
المصدر: Plant Biotechnology Journal ; volume 21, issue 12, page 2546-2559 ; ISSN 1467-7644 1467-7652
بيانات النشر: Wiley
سنة النشر: 2023
المجموعة: Wiley Online Library (Open Access Articles via Crossref)
الوصف: Summary Pestiviruses, including classical swine fever virus, remain a concern for global animal health and are responsible for major economic losses of livestock worldwide. Despite high levels of vaccination, currently available commercial vaccines are limited by safety concerns, moderate efficacy, and required high doses. The development of new vaccines is therefore essential. Vaccine efforts should focus on optimizing antigen presentation to enhance immune responses. Here, we describe a simple herringbone‐dimer strategy for efficient vaccine design, using the classical swine fever virus E2 expressed in a rice endosperm as an example. The expression of rE2 protein was identified, with the rE2 antigen accumulating to 480 mg/kg. Immunological assays in mice, rabbits, and pigs showed high antigenicity of rE2. Two immunizations with 284 ng of the rE2 vaccine or one shot with 5.12 μg provided effective protection in pigs without interference from pre‐existing antibodies. Crystal structure and small‐angle X‐ray scattering results confirmed the stable herringbone dimeric conformation, which had two fully exposed duplex receptor binding domains. Our results demonstrated that rice endosperm is a promising platform for precise vaccine design, and this strategy can be universally applied to other Flaviviridae virus vaccines.
نوع الوثيقة: article in journal/newspaper
اللغة: English
DOI: 10.1111/pbi.14152
الإتاحة: https://doi.org/10.1111/pbi.14152Test
حقوق: http://creativecommons.org/licenses/by-nc-nd/4.0Test/
رقم الانضمام: edsbas.5134B55C
قاعدة البيانات: BASE