التفاصيل البيبلوغرافية
| العنوان: |
Verproside, the Most Active Ingredient in YPL-001 Isolated from Pseudolysimachion rotundum var. subintegrum, Decreases Inflammatory Response by Inhibiting PKCδ Activation in Human Lung Epithelial Cells |
| المؤلفون: |
Eun Sol Oh, Hyung Won Ryu, Mun-Ock Kim, Jae-Won Lee, Yu Na Song, Ji-Yoon Park, Doo-Young Kim, Hyunju Ro, Jinhyuk Lee, Tae-Don Kim, Sung-Tae Hong, Su Ui Lee, Sei-Ryang Oh |
| المصدر: |
International Journal of Molecular Sciences; Volume 24; Issue 8; Pages: 7229 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2023 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
chronic obstructive pulmonary disease, P. rotundum var. subintegrum, iridoid, NF-κB, MUC5AC, EGR-1 |
| جغرافية الموضوع: |
agris |
| الوصف: |
Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory lung disease which causes breathing problems. YPL-001, consisting of six iridoids, has potent inhibitory efficacy against COPD. Although YPL-001 has completed clinical trial phase 2a as a natural drug for COPD treatment, the most effective iridoid in YPL-001 and its mechanism for reducing airway inflammation remain unclear. To find an iridoid most effectively reducing airway inflammation, we examined the inhibitory effects of the six iridoids in YPL-001 on TNF or PMA-stimulated inflammation (IL-6, IL-8, or MUC5AC) in NCI-H292 cells. Here, we show that verproside among the six iridoids most strongly suppresses inflammation. Both TNF/NF-κB-induced MUC5AC expression and PMA/PKCδ/EGR-1-induced IL-6/-8 expression are successfully reduced by verproside. Verproside also shows anti-inflammatory effects on a broad range of airway stimulants in NCI-H292 cells. The inhibitory effect of verproside on the phosphorylation of PKC enzymes is specific to PKCδ. Finally, in vivo assay using the COPD-mouse model shows that verproside effectively reduces lung inflammation by suppressing PKCδ activation and mucus overproduction. Altogether, we propose YPL-001 and verproside as candidate drugs for treating inflammatory lung diseases that act by inhibiting PKCδ activation and its downstream pathways. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
Molecular Pathology, Diagnostics, and Therapeutics; https://dx.doi.org/10.3390/ijms24087229Test |
| DOI: |
10.3390/ijms24087229 |
| الإتاحة: |
https://doi.org/10.3390/ijms24087229Test |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.50CA7BED |
| قاعدة البيانات: |
BASE |
