دورية أكاديمية
Towards phasing using high X-ray intensity
| العنوان: | Towards phasing using high X-ray intensity |
|---|---|
| المؤلفون: | Galli, Lorenzo, Son, Sang-Kil, Nass, Karol, Shoeman, Robert L., Timneanu, Nicusor, Santra, Robin, Schlichting, Ilme, Chapman, Henry N., Barends, Thomas R. M., White, Thomas, Barty, Anton, Botha, Sabine, Boutet, Sébastien, Caleman, Carl, Doak, R. Bruce, Nanao, Max H. |
| المصدر: | IUCrJ 2(6), 1-8 (2015). doi:10.1107/S2052252515014049 |
| بيانات النشر: | International Union of Crystallography (IUCr) |
| سنة النشر: | 2015 |
| المجموعة: | DESY Publication Database (PUBDB) |
| مصطلحات موضوعية: | info:eu-repo/classification/ddc/530 |
| جغرافية الموضوع: | DE |
| الوصف: | X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential ‘bleaching’ of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme isproposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/issn/2052-2525; info:eu-repo/semantics/altIdentifier/pmid/pmid:26594370; info:eu-repo/semantics/altIdentifier/wos/WOS:000364415900007; https://bib-pubdb1.desy.de/record/275862Test; https://bib-pubdb1.desy.de/search?p=id:%22PUBDB-2015-04279%22Test |
| الإتاحة: | https://doi.org/10.1107/S2052252515014049Test https://bib-pubdb1.desy.de/record/275862Test https://bib-pubdb1.desy.de/search?p=id:%22PUBDB-2015-04279%22Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.4F8929C4 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |