دورية أكاديمية
Modulating the chemo-mechanical response of structured DNA assemblies through binding molecules
| العنوان: | Modulating the chemo-mechanical response of structured DNA assemblies through binding molecules |
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| المؤلفون: | Lee, Chanseok, Kim, Young-Joo, Kim, Kyung Soo, Lee, Jae Young, Kim, Do-Nyun |
| المساهمون: | National Convergence Research of Scientific Challenges, Basic Research in Science & Engineering, National Research Foundation of Korea, Ministry of Science and ICT, South Korea |
| المصدر: | Nucleic Acids Research ; volume 49, issue 21, page 12591-12599 ; ISSN 0305-1048 1362-4962 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Genetics |
| الوصف: | Recent advances in DNA nanotechnology led the fabrication and utilization of various DNA assemblies, but the development of a method to control their global shapes and mechanical flexibilities with high efficiency and repeatability is one of the remaining challenges for the realization of the molecular machines with on-demand functionalities. DNA-binding molecules with intercalation and groove binding modes are known to induce the perturbation on the geometrical and mechanical characteristics of DNA at the strand level, which might be effective in structured DNA assemblies as well. Here, we demonstrate that the chemo-mechanical response of DNA strands with binding ligands can change the global shape and stiffness of DNA origami nanostructures, thereby enabling the systematic modulation of them by selecting a proper ligand and its concentration. Multiple DNA-binding drugs and fluorophores were applied to straight and curved DNA origami bundles, which demonstrated a fast, recoverable, and controllable alteration of the bending persistence length and the radius of curvature of DNA nanostructures. This chemo-mechanical modulation of DNA nanostructures would provide a powerful tool for reconfigurable and dynamic actuation of DNA machineries. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1093/nar/gkab1119 |
| الإتاحة: | https://doi.org/10.1093/nar/gkab1119Test https://academic.oup.com/nar/article-pdf/49/21/12591/41503895/gkab1119.pdfTest |
| حقوق: | https://creativecommons.org/licenses/by-nc/4.0Test/ |
| رقم الانضمام: | edsbas.4E992527 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/nar/gkab1119 |
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