Lytic polysaccharide monooxygenase increases cellobiohydrolases activity by promoting decrystallization of cellulose surface
| العنوان: | Lytic polysaccharide monooxygenase increases cellobiohydrolases activity by promoting decrystallization of cellulose surface |
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| المؤلفون: | Uchiyama, Taku, Uchihashi, Takayuki, Ishida, Takuya, Nakamura, Akihiko, Vermaas, Josh V., Crowley, Michael F., Samejima, Masahiro, Beckham, Gregg T., Igarashi, Kiyohiko |
| سنة النشر: | 2023 |
| المجموعة: | SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) |
| مصطلحات موضوعية: | 59 BASIC BIOLOGICAL SCIENCES, 09 BIOMASS FUELS |
| الوصف: | Efficient depolymerization of crystalline cellulose requires cooperation between multiple cellulolytic enzymes. Through biochemical approaches, molecular dynamics (MD) simulation, and single-molecule observations using high-speed atomic force microscopy (HS-AFM), we quantify and track synergistic activity for cellobiohydrolases (CBHs) with a lytic polysaccharide monooxygenase (LPMO) from Phanerochaete chrysosporium. Increasing concentrations of LPMO (AA9D) increased the activity of a glycoside hydrolase family 6 CBH, Cel6A, whereas the activity of a family 7 CBH (Cel7D) was enhanced only at lower concentrations of AA9D. MD simulation suggests that the result of AA9D action to produce chain breaks in crystalline cellulose can oxidatively disturb the crystalline surface by disrupting hydrogen bonds. HS-AFM observations showed that AA9D increased the number of Cel7D molecules moving on the substrate surface and increased the processivity of Cel7D, thereby increasing the depolymerization performance, suggesting that AA9D not only generates chain ends but also amorphizes the crystalline surface, thereby increasing the activity of CBHs. |
| نوع الوثيقة: | other/unknown material |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | http://www.osti.gov/servlets/purl/1923870Test; https://www.osti.gov/biblio/1923870Test; https://doi.org/10.1126/sciadv.ade5155Test |
| DOI: | 10.1126/sciadv.ade5155 |
| الإتاحة: | https://doi.org/10.1126/sciadv.ade5155Test http://www.osti.gov/servlets/purl/1923870Test https://www.osti.gov/biblio/1923870Test |
| رقم الانضمام: | edsbas.4E5B7F8 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1126/sciadv.ade5155 |
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