دورية أكاديمية
Capsules and their traits shape phage susceptibility and plasmid conjugation efficiency
| العنوان: | Capsules and their traits shape phage susceptibility and plasmid conjugation efficiency |
|---|---|
| المؤلفون: | Haudiquet, Matthieu, Le Bris, Julie, Nucci, Amandine, Bonnin, Rémy, A, Domingo-Calap, Pilar, Rocha, Eduardo, P C, Rendueles, Olaya |
| المساهمون: | Génomique évolutive des Microbes / Microbial Evolutionary Genomics, Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Ecole Doctorale Frontiere de l’Innovation en Recherche et Education (ED 474 FIRE), Université Paris Cité (UPCité)-Université Paris Sciences et Lettres (PSL), Ecole Doctorale Complexité du Vivant (ED515), Sorbonne Université (SU), Immunologie des maladies virales, auto-immunes, hématologiques et bactériennes (IMVA-HB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, Centre National de Référence Associé de la Résistance aux Antibiotiques Hôpital Bicêtre AP-HP (CNRARA/Service de Microbiologie), Hôpital Bicêtre AP-HP, Le Kremlin-Bicêtre, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service de Microbiologie Hôpital Bicêtre AP-HP (UBH : Unité de Bactériologie-Hygiène), Instituto de Biología Integrativa de Sistemas (I²SysBio), Universitat de València (UV), This work was supported by an ANR JCJC (Agence national de recherche) grant ANR 18 CE12 0001 01 ENCAPSULATION awarded to O.R. and by the grant ANR 16 CE15 0022 03 PREDIRES awarded to EPCR. The laboratory is funded by a Laboratoire d’Excellence ‘Integrative Biology of Emerging Infectious Diseases’ grant ANR-10-LABX-62- IBEID , the INCEPTION programme PIA/ANR-16- CONV-0005 , and the FRM EQU201903007835 . M.H. has received funding from the FIRE Doctoral School (Centre de Recherche Interdisciplinaire, programme Bettencourt) to attend conferences. P.D.-C. was financially supported by a Ramón y Cajal contract RYC2019-028015-I funded by MCIN/AEI/10.13039/501100011033, ESF Invest in your future., We thank Olivier Tenaillon and Nienke Buddelmeijer for fruitful discussions. We thank Sylvain Brisse for providing us with the Klebsiella strains. We thank Jean-Marc Ghigo and Christophe Beloin for the gift of plasmid R1-dr19. We thank Julia Bos for the gift of phage phK1. We are grateful to the members of the Microbial Evolutionary Genomics lab and Maxime Policarpo, Grégoire Haouy and Léone Debarge for helpful discussions. This work used the computational and storage services (TARS & MAESTRO cluster) provided by the IT department at Institut Pasteur, Paris, and the media preparation services provided by the Plateforme Milieux. The sequencing work was performed by the Biomics Platform, C2RT, Institut Pasteur, Paris, France, supported by France Géno- mique (ANR-10-INBS-09) and IBISA, ANR-18-CE12-0001,ENCAPSULATION,Le rôle évolutif des capsules dans l'adaptation bactérienne(2018), ANR-16-CE15-0022,PREDIRES,PREDIction de l'émergence de la RESistance bactérienne dans le microbiote intestinal humain lors d'un traitement antibiotique(2016), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-16-CONV-0005,INCEPTION,Institut Convergences pour l'étude de l'Emergence des Pathologies au Travers des Individus et des populatiONs(2016) |
| المصدر: | ISSN: 2041-1723. |
| بيانات النشر: | HAL CCSD Nature Publishing Group |
| سنة النشر: | 2024 |
| المجموعة: | HAL-CEA (Commissariat à l'énergie atomique et aux énergies alternatives) |
| مصطلحات موضوعية: | [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE], [SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis |
| الوصف: | International audience ; Abstract Bacterial evolution is affected by mobile genetic elements like phages and conjugative plasmids, offering new adaptive traits while incurring fitness costs. Their infection is affected by the bacterial capsule. Yet, its importance has been difficult to quantify because of the high diversity of confounding mechanisms in bacterial genomes such as anti-viral systems and surface receptor modifications. Swapping capsule loci between Klebsiella pneumoniae strains allowed us to quantify their impact on plasmid and phage infection independently of genetic background. Capsule swaps systematically invert phage susceptibility, revealing serotypes as key determinants of phage infection. Capsule types also influence conjugation efficiency in both donor and recipient cells, a mechanism shaped by capsule volume and conjugative pilus structure. Comparative genomics confirmed that more permissive serotypes in the lab correspond to the strains acquiring more conjugative plasmids in nature. The least capsule-sensitive pili (F-like) are the most frequent in the species’ plasmids, and are the only ones associated with both antibiotic resistance and virulence factors, driving the convergence between virulence and antibiotics resistance in the population. These results show how traits of cellular envelopes define slow and fast lanes of infection by mobile genetic elements, with implications for population dynamics and horizontal gene transfer. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/38448399; hal-04492345; https://hal.science/hal-04492345Test; https://hal.science/hal-04492345/documentTest; https://hal.science/hal-04492345/file/33-HaudiquetEtAl_NatCommuns41467-024-46147-5.pdfTest; PUBMED: 38448399 |
| DOI: | 10.1038/s41467-024-46147-5 |
| الإتاحة: | https://doi.org/10.1038/s41467-024-46147-5Test https://hal.science/hal-04492345Test https://hal.science/hal-04492345/documentTest https://hal.science/hal-04492345/file/33-HaudiquetEtAl_NatCommuns41467-024-46147-5.pdfTest |
| حقوق: | http://creativecommons.org/licenses/byTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.4C809188 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-024-46147-5 |
|---|