دورية أكاديمية
Interrogating Membrane Protein Conformational Dynamics within Native Lipid Compositions.
| العنوان: | Interrogating Membrane Protein Conformational Dynamics within Native Lipid Compositions. |
|---|---|
| المؤلفون: | Reading, Eamonn, Hall, Zoe, Martens, Chloé, Haghighi, Tabasom, Findlay, Heather, Ahdash, Zainab, Politis, Argyris, Booth, P. S L |
| المصدر: | Angewandte Chemie, 56 (49 |
| سنة النشر: | 2017 |
| المجموعة: | DI-fusion : dépôt institutionnel de l'Université libre de Bruxelles (ULB) |
| مصطلحات موضوعية: | Sciences bio-médicales et agricoles, DNA-Binding Proteins -- metabolism, Deuterium Exchange Measurement, Endopeptidases -- metabolism, Escherichia coli Proteins -- metabolism, Lipids -- chemistry, Mass Spectrometry, Membrane Proteins -- chemistry -- metabolism, Protein Conformation, lipids, membrane nanodiscs, membrane proteins, structural biology |
| الوصف: | The interplay between membrane proteins and the lipids of the membrane is important for cellular function, however, tools enabling the interrogation of protein dynamics within native lipid environments are scarce and often invasive. We show that the styrene-maleic acid lipid particle (SMALP) technology can be coupled with hydrogen-deuterium exchange mass spectrometry (HDX-MS) to investigate membrane protein conformational dynamics within native lipid bilayers. We demonstrate changes in accessibility and dynamics of the rhomboid protease GlpG, captured within three different native lipid compositions, and identify protein regions sensitive to changes in the native lipid environment. Our results illuminate the value of this approach for distinguishing the putative role(s) of the native lipid composition in modulating membrane protein conformational dynamics. ; info:eu-repo/semantics/published |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 1 full-text file(s): application/pdf |
| اللغة: | English |
| العلاقة: | uri/info:doi/10.1002/anie.201709657; uri/info:pmid/29049865; https://dipot.ulb.ac.be/dspace/bitstream/2013/310048/3/Reading_et_al2017Angewandte_Chemie.pdfTest; http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/310048Test |
| الإتاحة: | http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/310048Test https://dipot.ulb.ac.be/dspace/bitstream/2013/310048/3/Reading_et_al2017Angewandte_Chemie.pdfTest |
| رقم الانضمام: | edsbas.4AEA1C12 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |