دورية أكاديمية
Pharmacological Dissection of the Crosstalk between NaV and CaV Channels in GH3b6 Cells
| العنوان: | Pharmacological Dissection of the Crosstalk between NaV and CaV Channels in GH3b6 Cells |
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| المؤلفون: | Réthoré, Léa, Park, Joohee, Montnach, Jérôme, Nicolas, Sébastien, Khoury, Joseph, Le Seac’h, Elodie, Mabrouk, Kamel, de Pomyers, Harold, Tricoire-Leignel, Hélène, Mattei, César, Henrion, Daniel, Fajloun, Ziad, de Waard, Michel, Legendre, Claire, Legros, Christian |
| المساهمون: | MitoVasc - Équipe CarME (Cardiovascular Pathophysiology) (MITOVASC-CARME), MitoVasc - Physiopathologie Cardiovasculaire et Mitochondriale (MITOVASC), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut du Thorax Nantes, ITX-lab unité de recherche de l'institut du thorax UMR1087 UMR6291 (ITX-lab), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Nantes Université - UFR de Médecine et des Techniques Médicales (Nantes Univ - UFR MEDECINE), Nantes Université - pôle Santé, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Santé, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ), Institut de Chimie Radicalaire (ICR), Aix Marseille Université (AMU)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Azm Center for research in biotechnology and its applications TRIPOLI, Université Libanaise |
| المصدر: | ISSN: 1661-6596. |
| بيانات النشر: | HAL CCSD MDPI |
| سنة النشر: | 2022 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | GH3b6 cells, voltage-gated sodium channel, L-type voltage-gated calcium channel, veratridine, patch-clamp, Fura-2, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology |
| الوصف: | Thanks to the crosstalk between Na+ and Ca2+ channels, Na+ and Ca2+ homeostasis interplay in so-called excitable cells enables the generation of action potential in response to electrical stimulation. Here, we investigated the impact of persistent activation of voltage-gated Na+ (NaV) channels by neurotoxins, such as veratridine (VTD), on intracellular Ca2+ concentration ([Ca2+]i) in a model of excitable cells, the rat pituitary GH3b6 cells, in order to identify the molecular actors involved in Na+-Ca2+ homeostasis crosstalk. By combining RT-qPCR, immunoblotting, immunocytochemistry, and patch-clamp techniques, we showed that GH3b6 cells predominantly express the NaV1.3 channel subtype, which likely endorses their voltage-activated Na+ currents. Notably, these Na+ currents were blocked by ICA-121431 and activated by the β-scorpion toxin Tf2, two selective NaV1.3 channel ligands. Using Fura-2, we showed that VTD induced a [Ca2+]i increase. This effect was suppressed by the selective NaV channel blocker tetrodotoxin, as well by the selective L-type CaV channel (LTCC) blocker nifedipine. We also evidenced that crobenetine, a NaV channel blocker, abolished VTD-induced [Ca2+]i elevation, while it had no effects on LTCC. Altogether, our findings highlight a crosstalk between NaV and LTCC in GH3b6 cells, providing a new insight into the mode of action of neurotoxins. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-04215087; https://hal.science/hal-04215087Test; https://hal.science/hal-04215087/documentTest; https://hal.science/hal-04215087/file/ijms-23-00827.pdfTest |
| DOI: | 10.3390/ijms23020827 |
| الإتاحة: | https://doi.org/10.3390/ijms23020827Test https://hal.science/hal-04215087Test https://hal.science/hal-04215087/documentTest https://hal.science/hal-04215087/file/ijms-23-00827.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.4A4F1CD |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/ijms23020827 |
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