Hexachlorobenzene impairs glucose metabolism in a rat model of porphyria cutanea tarda: A mechanistic approach
| العنوان: | Hexachlorobenzene impairs glucose metabolism in a rat model of porphyria cutanea tarda: A mechanistic approach |
|---|---|
| المؤلفون: | Mazzetti, Marta Blanca, Taira, María Cristina, Lelli de Angeletti, Sandra Marcela |
| سنة النشر: | 2004 |
| المجموعة: | Biblioteca Digital FCEN-UBA (Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires) |
| مصطلحات موضوعية: | Gluconeogenesis, Glucose, Hexachlorobenzene, Insulin, Porphyria cutanea tarda, glucose 6 phosphatase, glycogen, phosphoenolpyruvate carboxykinase (GTP), pyruvate carboxylase, pyruvate kinase, animal cell, animal experiment, animal model, animal tissue, article, controlled study, evaluation, female, glucose blood level, glucose metabolism, glycogen liver level, insulin blood level, nonhuman, oxidative stress, priority journal, rat, Animals, Disease Models, Animal, Enzyme Inhibitors |
| الوصف: | Hexachlobenzene (HCB), one of the most persistent environmental pollutants, induces porphyria cutanea tarda (PCT). The aim of this work was to analyze the effect of HCB on some aspects of glucose metabolism, particularly those related to its neosynthesis in vivo. For this purpose, a time-course study on gluconeogenic enzymes, pyruvate carboxylase (PC), phosphoenolpyruvate carboxykinase (PEPCK), glucose-6-phosphatase (G-6-Pase) and on pyruvate kinase (PK), a glycolytic enzyme, was carried out. Plasma glucose and insulin levels, hepatic glycogen, tryptophan contents, and the pancreatic insulin secretion pattern stimulated by glucose were investigated. Oxidative stress and heme pathway parameters were also evaluated. HCB treatment decreased PC, PEPCK, and G-6-Pase activities. The effect was observed at an early time point and grew as the treatment progressed. Loss of 60, 56, and 37%, respectively, was noted at the end of the treatment when a considerable amount of porphyrins had accumulated in the liver as a result of drastic blockage of uroporphyrinogen decarboxylase (URO-D) (95% inhibition). The plasma glucose level was reduced (one-third loss), while storage of hepatic glucose was stimulated in a time-dependent way by HCB treatment. A decay in the normal plasma insulin level was observed as fungicide intoxication progressed (twice to four times lower). However, normal insulin secretion of perifused pancreatic Langerhans islets stimulated by glucose during the 3rd and 6th weeks of treatment did not prove to be significantly affected. HCB promoted a time-dependent increase in urinary chemiluminiscence (fourfold) and hepatic malondialdehide (MDA) content (fivefold), while the liver tryptophan level was only raised at the longest intoxication times. These results would suggest that HCB treatment does not cause a primary alteration in the mechanism of pancreatic insulin secretion and that the changes induced by the fungicide on insulin levels would be an adaptative response of the organism to stimulate ... |
| نوع الوثيقة: | other/unknown material |
| اللغة: | unknown |
| العلاقة: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03405761_v78_n1_p25_MazzettiTest; http://hdl.handle.net/20.500.12110/paper_03405761_v78_n1_p25_MazzettiTest |
| الإتاحة: | https://doi.org/20.500.12110/paper_03405761_v78_n1_p25_MazzettiTest https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03405761_v78_n1_p25_MazzettiTest https://hdl.handle.net/20.500.12110/paper_03405761_v78_n1_p25_MazzettiTest |
| رقم الانضمام: | edsbas.4A2ED2A6 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |